TY - JOUR
T1 - Structural and Functional Characterization of the Hazelnut Allergen Cor a 8
AU - Offermann, Lesa
AU - Bublin, Merima
AU - Perdue, Makenzie
AU - Pfeifer, Sabine
AU - Dubiela, Pawel
AU - Borowski, Tomasz
AU - Chruszcz, Maksymilian
AU - Hoffmann-Sommergruber, Karin
N1 - Publisher Copyright:
© 2015 American Chemical Society.
PY - 2015
Y1 - 2015
N2 - Nonspecific lipid transfer proteins (nsLTPs) are basic proteins, stabilized by four disulfide bonds, and are expressed throughout the plant kingdom. These proteins are also known as important allergens in fruits and tree nuts. In this study, the nsLTP from hazelnuts, Cor a 8, was purified and its crystal structure determined. The protein is stable at low pH and refolds after thermal denaturation. Molecular dynamics simulations were used to provide an insight into conformational changes of Cor a 8 upon ligand binding. When known epitope areas from Pru p 3 were compared to those of Cor a 8, differences were obvious, which may contribute to limited cross-reactivity between peach and hazelnut allergens. Differences in epitope regions may contribute to limited cross-reactivity between Cor a 8 and nsLTPs from other plant sources. The structure of Cor a 8 represents the first resolved structure of a hazelnut allergen.
AB - Nonspecific lipid transfer proteins (nsLTPs) are basic proteins, stabilized by four disulfide bonds, and are expressed throughout the plant kingdom. These proteins are also known as important allergens in fruits and tree nuts. In this study, the nsLTP from hazelnuts, Cor a 8, was purified and its crystal structure determined. The protein is stable at low pH and refolds after thermal denaturation. Molecular dynamics simulations were used to provide an insight into conformational changes of Cor a 8 upon ligand binding. When known epitope areas from Pru p 3 were compared to those of Cor a 8, differences were obvious, which may contribute to limited cross-reactivity between peach and hazelnut allergens. Differences in epitope regions may contribute to limited cross-reactivity between Cor a 8 and nsLTPs from other plant sources. The structure of Cor a 8 represents the first resolved structure of a hazelnut allergen.
UR - http://www.scopus.com/inward/record.url?scp=84945312371&partnerID=8YFLogxK
U2 - 10.1021/acs.jafc.5b03534
DO - 10.1021/acs.jafc.5b03534
M3 - Journal article
SN - 0021-8561
VL - 63
SP - 9150
EP - 9158
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
IS - 41
ER -