Quantitative PCR method for sensitive detection of ruminant fecal pollution in freshwater and evaluation of this method in alpine karstic regions

Georg H. Reischer, David C. Kasper, Ralf Steinborn, Robert L. Mach, Andreas H. Farnleitner*

*Corresponding author for this work

Research output: Journal article (peer-reviewed)Journal article

149 Citations (Scopus)

Abstract

A quantitative TaqMan minor-groove binder real-time PCR assay was developed for the sensitive detection of a ruminant-specific genetic marker in fecal members of the phylum Bacteroidetes. The qualitative and quantitative detection limits determined were 6 and 20 marker copies per PCR, respectively. Tested ruminant feces contained an average of 4.1 × 109 marker equivalents per g, allowing the detection of 1.7 ng of feces per filter in fecal suspensions. The marker was detected in water samples from a karstic catchment area at levels matching a gradient from negligible to considerable ruminant fecal influence (from not detectable to 105 marker equivalents per liter).

Original languageEnglish
Pages (from-to)5610-5614
Number of pages5
JournalApplied and Environmental Microbiology
Volume72
Issue number8
DOIs
Publication statusPublished - Aug 2006
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Applied Microbiology and Biotechnology
  • Ecology

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