Quantitative PCR method for sensitive detection of ruminant fecal pollution in freshwater and evaluation of this method in alpine karstic regions

Georg H. Reischer; David C. Kasper; Ralf Steinborn; Robert L. Mach; Andreas H. Farnleitner

doi:10.1128/AEM.00364-06

Quantitative PCR method for sensitive detection of ruminant fecal pollution in freshwater and evaluation of this method in alpine karstic regions

Georg H. Reischer, David C. Kasper, Ralf Steinborn, Robert L. Mach, Andreas H. Farnleitner^*

^*Corresponding author for this work

Research output: Journal article (peer-reviewed) › Journal article

159 Citations (Scopus)

Abstract

A quantitative TaqMan minor-groove binder real-time PCR assay was developed for the sensitive detection of a ruminant-specific genetic marker in fecal members of the phylum Bacteroidetes. The qualitative and quantitative detection limits determined were 6 and 20 marker copies per PCR, respectively. Tested ruminant feces contained an average of 4.1 × 10⁹ marker equivalents per g, allowing the detection of 1.7 ng of feces per filter in fecal suspensions. The marker was detected in water samples from a karstic catchment area at levels matching a gradient from negligible to considerable ruminant fecal influence (from not detectable to 10⁵ marker equivalents per liter).

Original language	English
Pages (from-to)	5610-5614
Number of pages	5
Journal	Applied and Environmental Microbiology
Volume	72
Issue number	8
DOIs	https://doi.org/10.1128/AEM.00364-06
Publication status	Published - Aug 2006
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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title = "Quantitative PCR method for sensitive detection of ruminant fecal pollution in freshwater and evaluation of this method in alpine karstic regions",

abstract = "A quantitative TaqMan minor-groove binder real-time PCR assay was developed for the sensitive detection of a ruminant-specific genetic marker in fecal members of the phylum Bacteroidetes. The qualitative and quantitative detection limits determined were 6 and 20 marker copies per PCR, respectively. Tested ruminant feces contained an average of 4.1 × 109 marker equivalents per g, allowing the detection of 1.7 ng of feces per filter in fecal suspensions. The marker was detected in water samples from a karstic catchment area at levels matching a gradient from negligible to considerable ruminant fecal influence (from not detectable to 105 marker equivalents per liter).",

author = "Reischer, {Georg H.} and Kasper, {David C.} and Ralf Steinborn and Mach, {Robert L.} and Farnleitner, {Andreas H.}",

year = "2006",

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AU - Reischer, Georg H.

AU - Kasper, David C.

AU - Steinborn, Ralf

AU - Mach, Robert L.

AU - Farnleitner, Andreas H.

PY - 2006/8

Y1 - 2006/8

N2 - A quantitative TaqMan minor-groove binder real-time PCR assay was developed for the sensitive detection of a ruminant-specific genetic marker in fecal members of the phylum Bacteroidetes. The qualitative and quantitative detection limits determined were 6 and 20 marker copies per PCR, respectively. Tested ruminant feces contained an average of 4.1 × 109 marker equivalents per g, allowing the detection of 1.7 ng of feces per filter in fecal suspensions. The marker was detected in water samples from a karstic catchment area at levels matching a gradient from negligible to considerable ruminant fecal influence (from not detectable to 105 marker equivalents per liter).

AB - A quantitative TaqMan minor-groove binder real-time PCR assay was developed for the sensitive detection of a ruminant-specific genetic marker in fecal members of the phylum Bacteroidetes. The qualitative and quantitative detection limits determined were 6 and 20 marker copies per PCR, respectively. Tested ruminant feces contained an average of 4.1 × 109 marker equivalents per g, allowing the detection of 1.7 ng of feces per filter in fecal suspensions. The marker was detected in water samples from a karstic catchment area at levels matching a gradient from negligible to considerable ruminant fecal influence (from not detectable to 105 marker equivalents per liter).

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SN - 0099-2240

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SP - 5610

EP - 5614

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 8

ER -

Quantitative PCR method for sensitive detection of ruminant fecal pollution in freshwater and evaluation of this method in alpine karstic regions

Abstract

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