Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan probe

G. H. Reischer; M. Lemmens; A. Farnleitner; A. Adler; R. L. Mach

doi:10.1016/j.mimet.2004.06.003

Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan probe

G. H. Reischer
, M. Lemmens
, A. Farnleitner
, A. Adler
, R. L. Mach^*

^*Corresponding author for this work

Research output: Journal article (peer-reviewed) › Journal article

82 Citations (Scopus)

Abstract

A new real-time PCR based method was developed for the species-specific detection, identification and quantification of Fusarium graminearum in planta. It utilizes a TaqMan hybridisation probe targeting the beta-tubulin gene and a plasmid standard. The assay is highly specific giving no product with DNA of closely related species. It is very sensitive, detecting down to five gene copies per reaction, and is able to produce reliable quantitative data over a range of six orders of magnitude.

Original language	English
Pages (from-to)	141-146
Number of pages	6
Journal	Journal of Microbiological Methods
Volume	59
Issue number	1
DOIs	https://doi.org/10.1016/j.mimet.2004.06.003
Publication status	Published - Oct 2004
Externally published	Yes

Keywords

Fusarium graminearum
Infected wheat
Real-time PCR
TaqMan Probe

ASJC Scopus subject areas

Microbiology
Molecular Biology
Microbiology (medical)

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10.1016/j.mimet.2004.06.003

Cite this

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title = "Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan probe",

abstract = "A new real-time PCR based method was developed for the species-specific detection, identification and quantification of Fusarium graminearum in planta. It utilizes a TaqMan hybridisation probe targeting the beta-tubulin gene and a plasmid standard. The assay is highly specific giving no product with DNA of closely related species. It is very sensitive, detecting down to five gene copies per reaction, and is able to produce reliable quantitative data over a range of six orders of magnitude.",

keywords = "Fusarium graminearum, Infected wheat, Real-time PCR, TaqMan Probe",

author = "Reischer, \{G. H.\} and M. Lemmens and A. Farnleitner and A. Adler and Mach, \{R. L.\}",

note = "Funding Information: This study was supported by grants from the Austrian Federal Ministry for Education, Science and Culture GEN-AU pilotproject (GZ 200.051/2-VI/1/2003) and from the Austrian Federal Ministry of Agriculture, Forestry, Environment and Water Management (FP 1264) to RLM.",

year = "2004",

month = oct,

doi = "10.1016/j.mimet.2004.06.003",

language = "English",

volume = "59",

pages = "141--146",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan probe

AU - Reischer, G. H.

AU - Lemmens, M.

AU - Farnleitner, A.

AU - Adler, A.

AU - Mach, R. L.

N1 - Funding Information: This study was supported by grants from the Austrian Federal Ministry for Education, Science and Culture GEN-AU pilotproject (GZ 200.051/2-VI/1/2003) and from the Austrian Federal Ministry of Agriculture, Forestry, Environment and Water Management (FP 1264) to RLM.

PY - 2004/10

Y1 - 2004/10

N2 - A new real-time PCR based method was developed for the species-specific detection, identification and quantification of Fusarium graminearum in planta. It utilizes a TaqMan hybridisation probe targeting the beta-tubulin gene and a plasmid standard. The assay is highly specific giving no product with DNA of closely related species. It is very sensitive, detecting down to five gene copies per reaction, and is able to produce reliable quantitative data over a range of six orders of magnitude.

AB - A new real-time PCR based method was developed for the species-specific detection, identification and quantification of Fusarium graminearum in planta. It utilizes a TaqMan hybridisation probe targeting the beta-tubulin gene and a plasmid standard. The assay is highly specific giving no product with DNA of closely related species. It is very sensitive, detecting down to five gene copies per reaction, and is able to produce reliable quantitative data over a range of six orders of magnitude.

KW - Fusarium graminearum

KW - Infected wheat

KW - Real-time PCR

KW - TaqMan Probe

UR - https://www.scopus.com/pages/publications/4344715587

U2 - 10.1016/j.mimet.2004.06.003

DO - 10.1016/j.mimet.2004.06.003

M3 - Journal article

C2 - 15325762

AN - SCOPUS:4344715587

SN - 0167-7012

VL - 59

SP - 141

EP - 146

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

IS - 1

ER -

Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan probe

Abstract

Keywords

ASJC Scopus subject areas

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