Quantification of Fusarium graminearum in infected wheat by species specific real-time PCR applying a TaqMan probe

G. H. Reischer, M. Lemmens, A. Farnleitner, A. Adler, R. L. Mach*

*Corresponding author for this work

Research output: Journal article (peer-reviewed)Journal article

80 Citations (Scopus)

Abstract

A new real-time PCR based method was developed for the species-specific detection, identification and quantification of Fusarium graminearum in planta. It utilizes a TaqMan hybridisation probe targeting the beta-tubulin gene and a plasmid standard. The assay is highly specific giving no product with DNA of closely related species. It is very sensitive, detecting down to five gene copies per reaction, and is able to produce reliable quantitative data over a range of six orders of magnitude.

Original languageEnglish
Pages (from-to)141-146
Number of pages6
JournalJournal of Microbiological Methods
Volume59
Issue number1
DOIs
Publication statusPublished - Oct 2004
Externally publishedYes

Keywords

  • Fusarium graminearum
  • Infected wheat
  • Real-time PCR
  • TaqMan Probe

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology
  • Microbiology (medical)

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