TY - JOUR
T1 - Pru du 1, the Bet v 1-homologue from almond, is a major allergen in patients with birch pollen associated almond allergy
AU - Kabasser, Stefan
AU - Crvenjak, Nadja
AU - Schmalz, Stefanie
AU - Kalic, Tanja
AU - Hafner, Christine
AU - Dubiela, Pawel
AU - Kucharczyk, Aleksandra
AU - Bazan-Socha, Stanislawa
AU - Lukaszyk, Mateusz
AU - Breiteneder, Heimo
AU - Radauer, Christian
AU - Bublin, Merima
N1 - Funding Information:
This research was supported using resources of the VetCore Facility (Proteomics) of the University of Veterinary Medicine Vienna. We thank Ryosuke Nakamura (National Institute of Health Sciences, Tokyo, Japan) for kindly providing RS‐ATL8 cells for basophil activation assays. This work was supported by funds of the Oesterreichische Nationalbank (Austrian Central Bank), Anniversary Fund, project number: 17560 and the Austrian Science Fund (FWF) grant P 30936‐B30, and the Danube Allergy Research Cluster project P06 funded by the Country of Lower Austria.
Funding Information:
This research was supported using resources of the VetCore Facility (Proteomics) of the University of Veterinary Medicine Vienna. We thank Ryosuke Nakamura (National Institute of Health Sciences, Tokyo, Japan) for kindly providing RS-ATL8 cells for basophil activation assays. This work was supported by funds of the Oesterreichische Nationalbank (Austrian Central Bank), Anniversary Fund, project number: 17560 and the Austrian Science Fund (FWF) grant P 30936-B30, and the Danube Allergy Research Cluster project P06 funded by the Country of Lower Austria.
Publisher Copyright:
© 2022 The Authors. Clinical and Translational Allergy published by John Wiley & Sons Ltd on behalf of European Academy of Allergy and Clinical Immunology.
PY - 2022/8
Y1 - 2022/8
N2 - Background: Almond allergy is common and can manifest in two different forms. Primary almond allergy has been reported to be associated with sensitization to almond legumin Pru du 6. In birchendemic regions, there is a link between birch-pollinosis which is likely based on a cross-reactive Bet v 1 homologue, a yet unidentified allergen in almond. Therefore, we sought to identify and characterize a Bet v 1-homologue in almond.Methods: The expression of a Bet v 1 homologue in almond kernels was confirmed by mass spectrometry. The recombinant protein was produced in Escherichia coli and its cross-reactivity and allergenic potency was analyzed by IgE quantitative and competitive ELISA, immunoblotting and basophil histamine release using sera from 17 almond allergic patients.Results: The identified Bet v 1 homologue received the designation Pru du 1.0101. Pru du 1.0101 bound IgE from 82 % of almond allergic patients. Bet v 1 was able to inhibit IgE-binding to rPru du 1 by 100%, while rPru du 1 inhibited IgE binding to rBet v 1 by 48%. Pru du 1.0101 activated basophils, though 100- to 1000-fold higher concentrations were required for maximum activation in comparison to rBet v 1.Conclusion: Considering the strong inhibition capacity and higher allergenic potency of Bet v 1, the results provide compelling evidence for primary sensitization to Bet v 1 in case of birch pollen associated almond allergy. Combining Pru du 6 and Pru du 1 in diagnostic approaches may help to discriminate between primary and birch-pollen associated almond allergy.
AB - Background: Almond allergy is common and can manifest in two different forms. Primary almond allergy has been reported to be associated with sensitization to almond legumin Pru du 6. In birchendemic regions, there is a link between birch-pollinosis which is likely based on a cross-reactive Bet v 1 homologue, a yet unidentified allergen in almond. Therefore, we sought to identify and characterize a Bet v 1-homologue in almond.Methods: The expression of a Bet v 1 homologue in almond kernels was confirmed by mass spectrometry. The recombinant protein was produced in Escherichia coli and its cross-reactivity and allergenic potency was analyzed by IgE quantitative and competitive ELISA, immunoblotting and basophil histamine release using sera from 17 almond allergic patients.Results: The identified Bet v 1 homologue received the designation Pru du 1.0101. Pru du 1.0101 bound IgE from 82 % of almond allergic patients. Bet v 1 was able to inhibit IgE-binding to rPru du 1 by 100%, while rPru du 1 inhibited IgE binding to rBet v 1 by 48%. Pru du 1.0101 activated basophils, though 100- to 1000-fold higher concentrations were required for maximum activation in comparison to rBet v 1.Conclusion: Considering the strong inhibition capacity and higher allergenic potency of Bet v 1, the results provide compelling evidence for primary sensitization to Bet v 1 in case of birch pollen associated almond allergy. Combining Pru du 6 and Pru du 1 in diagnostic approaches may help to discriminate between primary and birch-pollen associated almond allergy.
UR - http://www.scopus.com/inward/record.url?scp=85136907266&partnerID=8YFLogxK
U2 - 10.1002/clt2.12177
DO - 10.1002/clt2.12177
M3 - Journal article
C2 - 35949989
SN - 2045-7022
VL - 12
SP - e12177
JO - Clinical and Translational Allergy
JF - Clinical and Translational Allergy
IS - 8
M1 - e12177
ER -