Probabilistic fecal pollution source profiling and microbial source tracking for an urban river catchment

Julia Derx, H Seda Kılıç, Rita Linke, Sílvia Cervero-Aragó, Christina Frick, Jack Schijven, Alexander K T Kirschner, Gerhard Lindner, Julia Walochnik, Gabrielle Stalder, Regina Sommer, Ernis Saracevic, Matthias Zessner, Alfred P Blaschke, Andreas H Farnleitner

Research output: Journal article (peer-reviewed)Journal article

Abstract

We developed an innovative approach to estimate the extent of fecal pollution sources for urban river catchments. The methodology consists of 1) catchment surveys complemented by literature data where needed for probabilistic estimates of daily produced fecal indicator (FIBs, E. coli, enterococci) and zoonotic reference pathogen numbers (Campylobacter, Cryptosporidium and Giardia) excreted by human and animal sources in a river catchment, 2) generating a hypothesis about the dominant sources of fecal pollution and selecting a source targeted monitoring design, and 3) verifying the results by comparing measured concentrations of chemical tracers, C. perfringens, and host-associated genetic microbial source tracking (MST) markers in the river, and by multi-parametric correlation analysis. We tested the approach at a study area in Vienna, Austria. The daily produced microbial particle numbers according to the probabilistic estimates indicated that, for the dry weather scenario, the discharge of treated wastewater (WWTP) was the primary contributor to fecal pollution. For the wet weather scenario, 80-99 % of the daily produced FIBs and pathogens resulted from combined sewer overflows (CSOs) according to the probabilistic estimates. When testing our hypothesis in the river, the measured concentrations of the human genetic fecal marker were log10 4 higher than for selected animal genetic fecal markers. Our analyses showed for the first-time statistical relationships between C. perfringens and a human genetic fecal marker (i.e. HF183/BacR287) with the reference pathogen Giardia in river water (Spearman rank correlation: 0.78-0.83, p < 0.05. The developed approach facilitates urban water safety management and provides a robust basis for microbial fate and transport models and microbial infection risk assessment.

Original languageEnglish
Article number159533
Pages (from-to)159533
JournalScience of the Total Environment
Volume857
Issue numberPt 2
DOIs
Publication statusPublished - 20 Jan 2023

Keywords

  • Advanced catchment survey
  • Fecal indicators
  • Informed choice of parameters
  • Microbial source tracking
  • Microbiological water safety
  • Micropollutants
  • Probabilistic modelling
  • Recreational water quality
  • Zoonotic reference pathogens

ASJC Scopus subject areas

  • Environmental Engineering
  • Environmental Chemistry
  • Waste Management and Disposal
  • Pollution

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