Preclinical In Vitro and In Vivo Evaluation of [18F]FE@SUPPY for Cancer PET Imaging: Limitations of a Xenograft Model for Colorectal Cancer

T Balber, J Singer, N Berroterán-Infante, M Dumanic, L Fetty, J Fazekas-Singer, C Vraka, L Nics, M Bergmann, K Pallitsch, H Spreitzer, W Wadsak, M Hacker, E Jensen-Jarolim, H Viernstein, M Mitterhauser

Research output: Journal article (peer-reviewed)Journal article

2 Citations (Scopus)

Abstract

Molecular imaging probes such as PET-tracers have the potential to improve the accuracy of tumor characterization by directly visualizing the biochemical situation. Thus, molecular changes can be detected early before morphological manifestation. The A3 adenosine receptor (A3AR) is described to be highly expressed in colon cancer cell lines and human colorectal cancer (CRC), suggesting this receptor as a tumor marker. The aim of this preclinical study was the evaluation of [18F]FE@SUPPY as a PET-tracer for CRC using in vitro imaging and in vivo PET imaging. First, affinity and selectivity of FE@SUPPY and its metabolites were determined, proving the favorable binding profile of FE@SUPPY. The human adenocarcinoma cell line HT-29 was characterized regarding its hA3AR expression and was subsequently chosen as tumor graft. Promising results regarding the potential of [18F]FE@SUPPY as a PET-tracer for CRC imaging were obtained by autoradiography as ≥2.3-fold higher accumulation of [18F]FE@SUPPY was found in CRC tissue compared to adjacent healthy colon tissue from the same patient. Nevertheless, first in vivo studies using HT-29 xenografts showed insufficient tumor uptake due to (1) poor conservation of target expression in xenografts and (2) unfavorable pharmacokinetics of [18F]FE@SUPPY in mice. We therefore conclude that HT-29 xenografts are not adequate to visualize hA3ARs using [18F]FE@SUPPY.

Original languageEnglish
Article number1269830
Pages (from-to)1269830
JournalContrast Media and Molecular Imaging
Volume2018
DOIs
Publication statusPublished - 2018

Keywords

  • Animals
  • Colorectal Neoplasms/diagnostic imaging
  • Fluorine Radioisotopes
  • HT29 Cells
  • Heterografts
  • Humans
  • Mice
  • Molecular Imaging/methods
  • Neoplasm Proteins/analysis
  • Nicotinic Acids/pharmacokinetics
  • Positron-Emission Tomography/methods
  • Radiopharmaceuticals/pharmacokinetics
  • Receptor, Adenosine A3/analysis

ASJC Scopus subject areas

  • Radiology, Nuclear Medicine and Imaging

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