Pharmacologic targeting of a stem/progenitor population in vivo is associated with enhanced bone regeneration in mice

Siddhartha Mukherjee, Noopur Raje, Jesse A. Schoonmaker, Julie C. Liu, Teru Hideshima, Marc N. Wein, Dallas C. Jones, Sonia Vallet, Mary L. Bouxsein, Samantha Pozzi, Shweta Chhetri, Y. David Seo, Joshua P. Aronson, Chirayu Patel, Mariateresa Fulciniti, Louise E. Purton, Laurie H. Glimcher, Jane B. Lian, Gary Stein, Kenneth C. AndersonDavid T. Scadden*

*Corresponding author for this work

Research output: Journal article (peer-reviewed)Journal article

216 Citations (Scopus)


Drug targeting of adult stem cells has been proposed as a strategy for regenerative medicine, but very few drugs are known to target stem cell populations in vivo. Mesenchymal stem/progenitor cells (MSCs) are a multipotent population of cells that can differentiate into muscle, bone, fat, and other cell types in context-specific manners. Bortezomib (Bzb) is a clinically available proteasome inhibitor used in the treatment of multiple myeloma. Here, we show that Bzb induces MSCs to preferentially undergo osteoblastic differentiation, in part by modulation of the bone-specifying transcription factor runt-related transcription factor 2 (Runx-2) in mice. Mice implanted with MSCs showed increased ectopic ossicle and bone formation when recipients received low doses of Bzb. Furthermore, this treatment increased bone formation and rescued bone loss in a mouse model of osteoporosis. Thus, we show that a tissue-resident adult stem cell population in vivo can be pharmacologically modified to promote a regenerative function in adult animals.

Original languageEnglish
Pages (from-to)491-504
Number of pages14
JournalJournal of Clinical Investigation
Issue number2
Publication statusPublished - 01 Feb 2008
Externally publishedYes

ASJC Scopus subject areas

  • Medicine (all)


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