Optoelectrophysiological stimulation of the human eye using fundus-controlled silent substitution technique

Sascha Klee, Dietmar Link, Patrick Bessler, Jens Haueisen

Research output: Journal article (peer-reviewed)Journal article

6 Citations (Scopus)


We design, characterize, and apply a novel optoelectrophysiological setup for a fundus-controlled silent substitution technique that accounts for interindividual variability in retina morphology and simultaneously monitors the stimulation site under investigation. We connect a digital color liquid crystal on silicon projector, an electron-multiplying imager, and a light-emitting diode to a fundus camera. The temporal and spatial characterization reveal a maximal contrast loss of 7 for the highest stimulation frequency (30 Hz) and maximum cutoff spatial frequencies of ∼120 cyclesdeg. Two silent substitution flash sequences are applied to modulate selective activity in the short-wavelength-sensitive cone (S-cone) and combined long- and middle-wavelength-sensitive cone (LM-cone) pathways. Simultaneously, the visual evoked potentials are recorded. The data are compared to the grand average responses from a previous study that employed standard computer-screen presentation and showed very good latency matches. All the volunteers in the present examination exhibit differences between the S-cone and LM-cone evoked potentials (parameters mean values: peak-to-peak amplitude, N1 latency, and P1 latency for S-coneLM-cone responses: 8 μV15 μV, 113 ms89 ms, 170 ms143 ms). We demonstrate that the developed optoelectrophysiological setup simultaneously provides imaging, functional stimulation, and electrophysiological investigation of the retina.

Original languageEnglish
Article number015002
Pages (from-to)015002
JournalJournal of Biomedical Optics
Issue number1
Publication statusPublished - 01 Jan 2011
Externally publishedYes


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