Molecular reactivity profiling upon immunotherapy with a 300 IR sublingual house dust mite tablet reveals marked humoral changes towards major allergens

Ekaterina Potapova, Véronique Bordas-Le Floch, Thomas Schlederer, Susanne Vrtala, Huey-Jy Huang, Giorgio W Canonica, Rudolf Valenta, Paolo M Matricardi, Laurent Mascarell

Research output: Journal article (peer-reviewed)Journal article

1 Citation (Scopus)

Abstract

BACKGROUND: Molecular antibody reactivity profiles have not yet been studied in depth in patients treated by sublingual house dust mite (HDM) tablet immunotherapy. Humoral immune responses to a large panel of HDM mite allergens were studied using allergen microarray technology in a subset of clinically defined high and low responder patients from a double-blind placebo-controlled allergen-specific immunotherapy (AIT) trial using sublingual 300 IR HDM tablets.

METHODS: Serum levels of IgE, IgG and IgG4 to 13 Dermatophagoides pteronyssinus molecules were measured at baseline and after 1-year AIT, using allergen microarrays in 100 subjects exhibiting high or low clinical benefit.

RESULTS: Der p 1, Der p 2 and Der p 23 were the most frequently recognized allergens in the study population. Patients with HDM-related asthma had significantly higher allergen-specific IgE levels to Der p 1 and Der p 23. No significant difference in the distribution of allergen sensitization pattern was observed between high and low responders. An increase in serum allergen-specific IgG and IgG4 occurred upon AIT, in particular to allergens Der p 1, Der p 2 and Der p 23 (p < 0.0001).

CONCLUSIONS: We confirm for our study population that Der p 1- and Der p 23-specific IgE levels are associated with asthma. IgE reactivity profiles were not predicitive of sublingual AIT outcomes, with 300 IR tablets as efficacious in pauci- and multi-sensitized subjects. Our study is the first to demonstrate the induction of IgG and IgG4 specific for the HDM allergens Der p 1, Der p 2 and Der p 23 by sublingual AIT.

Original languageEnglish
Pages (from-to)3084-3095
Number of pages12
JournalAllergy: European Journal of Allergy and Clinical Immunology
Volume77
Issue number10
Early online date26 Apr 2022
DOIs
Publication statusPublished - Oct 2022

Keywords

  • SLIT tablet
  • allergen microarray
  • allergen-specific immunotherapy
  • house dust mite allergy
  • molecular reactivity profile
  • Allergens
  • Immunologic Factors
  • Tablets
  • Humans
  • Immunoglobulin G
  • Immunoglobulin E
  • Sublingual Immunotherapy
  • Antigens, Dermatophagoides
  • Pyridinolcarbamate
  • Animals
  • Asthma/therapy
  • Pyroglyphidae

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint

Dive into the research topics of 'Molecular reactivity profiling upon immunotherapy with a 300 IR sublingual house dust mite tablet reveals marked humoral changes towards major allergens'. Together they form a unique fingerprint.

Cite this