TY - JOUR
T1 - Molecular IgE sensitization profiles of urban and rural children in South Africa
AU - Mittermann, Irene
AU - Dzoro, Sheron
AU - Gattinger, Pia
AU - Botha, Maresa
AU - Basera, Wisdom
AU - Facey-Thomas, Heidi E
AU - Gaunt, Ben
AU - Genuneit, Jon
AU - Gray, Claudia L
AU - Hlela, Carol
AU - Flicker, Sabine
AU - Lunjani, Nonhlanhla
AU - Mankahla, Avumile
AU - Ramjith, Jordache
AU - Valenta, Rudolf
AU - Levin, Michael E
N1 - Publisher Copyright:
© 2020 European Academy of Allergy and Clinical Immunology and John Wiley & Sons Ltd
PY - 2021/2
Y1 - 2021/2
N2 - BACKGROUND: Allergens can act as disease-triggering factors in atopic dermatitis (AD) patients. The aim of the study was to elucidate the molecular IgE sensitization profile in children with and without AD living in urban and rural areas of South Africa.METHODS: Specific IgE reactivity was assessed in 166 Black South African children aged 9-38 months using a comprehensive panel of microarrayed allergens. According to clinical characterization children fell in four groups, urban AD cases (n = 32), urban controls (non-AD, n = 40), rural cases (n = 49) and rural controls (non-AD, n = 45).RESULTS: IgE reactivity to at least one of the allergens was detected in 94% of urban and 86% of rural AD children. House dust mite (HDM; 81% urban, 74% rural AD) and animal-derived allergens (50% urban, 31% rural AD) were the most frequently recognized respiratory allergens, whereas IgE to pollen allergens was almost absent. Urban AD children showed significantly higher frequency of IgE reactivity (50%) to mouse lipocalin, Mus m 1, than rural AD children (12%). The most frequently recognized food allergens were from egg (63% urban, 43% rural AD), peanut (31% vs 41%), and soybean (22% vs 27%), whereas milk sensitization was rare. α-gal-specific IgE almost exclusively occurred in rural children (AD: 14%, non-AD: 49%).CONCLUSION: Molecular allergy diagnosis detects frequent IgE sensitization to HDM, animal but not pollen allergens and to egg, peanut, and soy, but not milk allergens in African AD children. Urban AD children reacted more often to Mus m 1, whereas α-gal sensitization is more common in rural children likely due to parasite exposure.
AB - BACKGROUND: Allergens can act as disease-triggering factors in atopic dermatitis (AD) patients. The aim of the study was to elucidate the molecular IgE sensitization profile in children with and without AD living in urban and rural areas of South Africa.METHODS: Specific IgE reactivity was assessed in 166 Black South African children aged 9-38 months using a comprehensive panel of microarrayed allergens. According to clinical characterization children fell in four groups, urban AD cases (n = 32), urban controls (non-AD, n = 40), rural cases (n = 49) and rural controls (non-AD, n = 45).RESULTS: IgE reactivity to at least one of the allergens was detected in 94% of urban and 86% of rural AD children. House dust mite (HDM; 81% urban, 74% rural AD) and animal-derived allergens (50% urban, 31% rural AD) were the most frequently recognized respiratory allergens, whereas IgE to pollen allergens was almost absent. Urban AD children showed significantly higher frequency of IgE reactivity (50%) to mouse lipocalin, Mus m 1, than rural AD children (12%). The most frequently recognized food allergens were from egg (63% urban, 43% rural AD), peanut (31% vs 41%), and soybean (22% vs 27%), whereas milk sensitization was rare. α-gal-specific IgE almost exclusively occurred in rural children (AD: 14%, non-AD: 49%).CONCLUSION: Molecular allergy diagnosis detects frequent IgE sensitization to HDM, animal but not pollen allergens and to egg, peanut, and soy, but not milk allergens in African AD children. Urban AD children reacted more often to Mus m 1, whereas α-gal sensitization is more common in rural children likely due to parasite exposure.
KW - Allergens
KW - Animals
KW - Child
KW - Dermatitis, Atopic
KW - Food Hypersensitivity
KW - Humans
KW - Immunoglobulin E
KW - Mice
KW - South Africa/epidemiology
UR - http://www.scopus.com/inward/record.url?scp=85092377902&partnerID=8YFLogxK
U2 - 10.1111/pai.13377
DO - 10.1111/pai.13377
M3 - Journal article
C2 - 32969537
SN - 0905-6157
VL - 32
SP - 234
EP - 241
JO - Pediatric Allergy and Immunology
JF - Pediatric Allergy and Immunology
IS - 2
ER -