Modeling the conversion between specific IgE test platforms for nut allergens in children and adolescents

Jennifer A Hoang, Alper Celik, Christian Lupinek, Rudolf Valenta, Lucy Duan, Ruixue Dai, May G Brydges, Aimée Dubeau, Claire Lépine, Samantha Wong, Mara Alexanian-Farr, Ahuva Magder, Padmaja Subbarao, Julia E M Upton, Klara Schmidthaler, Zsolt Szépfalusi, Arun Ramani, Thomas Eiwegger

Research output: Journal article (peer-reviewed)Journal article

13 Citations (Scopus)


BACKGROUND: Multiplex tests allow for measurement of allergen-specific IgE responses to multiple extracts and molecular allergens and have several advantages for large cohort studies. Due to significant methodological differences, test systems are difficult to integrate in meta-analyses/systematic reviews since there is a lack of datasets with direct comparison. We aimed to create models for statistical integration of allergen-specific IgE to peanut/tree nut allergens from three IgE test platforms.

METHODS: Plasma from Canadian and Austrian children/adolescents with peanut/tree nut sensitization and a cohort of sensitized, high-risk, pre-school asthmatics (total n = 166) were measured with three R&D multiplex IgE test platforms: Allergy Explorer version 1 (ALEX) (Macro Array Dx), MeDALL-chip (Mechanisms of Development of Allergy) (Thermo Fisher), and EUROLINE (EUROIMMUN). Skin prick test (n = 51) and ImmunoCAP (Thermo Fisher) (n = 62) results for extracts were available in a subset. Regression models (Multivariate Adaptive Regression Splines, local polynomial regression) were applied if >30% of samples were positive to the allergen. Intra-test correlations between PR-10 and nsLTP allergens were assessed.

RESULTS: Using two regression methods, we demonstrated the ability to model allergen-specific relationships with acceptable measures of fit (r2 = 94%-56%) for peanut and tree nut sIgE testing at the extract and molecular-level, in order from highest to lowest: Ara h 2, Ara h 6, Jug r 1, Ana o 3, Ara h 1, Jug r 2, and Cor a 9.

CONCLUSION: Our models support the notion that quantitative conversion is possible between sIgE multiplex platforms for extracts and molecular allergens and may provide options to aggregate data for future meta-analysis.

Original languageEnglish
Pages (from-to)831-841
Number of pages11
JournalAllergy: European Journal of Allergy and Clinical Immunology
Issue number3
Publication statusPublished - Mar 2021


  • Adolescent
  • Allergens
  • Antigens, Plant
  • Arachis
  • Austria
  • Canada
  • Child
  • Humans
  • Immunoglobulin E
  • Nuts
  • Peanut Hypersensitivity


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