@article{9579665665054775a3f7179cca1bf82d,
title = "Modeling the conversion between specific IgE test platforms for nut allergens in children and adolescents",
abstract = "BACKGROUND: Multiplex tests allow for measurement of allergen-specific IgE responses to multiple extracts and molecular allergens and have several advantages for large cohort studies. Due to significant methodological differences, test systems are difficult to integrate in meta-analyses/systematic reviews since there is a lack of datasets with direct comparison. We aimed to create models for statistical integration of allergen-specific IgE to peanut/tree nut allergens from three IgE test platforms.METHODS: Plasma from Canadian and Austrian children/adolescents with peanut/tree nut sensitization and a cohort of sensitized, high-risk, pre-school asthmatics (total n = 166) were measured with three R&D multiplex IgE test platforms: Allergy Explorer version 1 (ALEX) (Macro Array Dx), MeDALL-chip (Mechanisms of Development of Allergy) (Thermo Fisher), and EUROLINE (EUROIMMUN). Skin prick test (n = 51) and ImmunoCAP (Thermo Fisher) (n = 62) results for extracts were available in a subset. Regression models (Multivariate Adaptive Regression Splines, local polynomial regression) were applied if >30% of samples were positive to the allergen. Intra-test correlations between PR-10 and nsLTP allergens were assessed.RESULTS: Using two regression methods, we demonstrated the ability to model allergen-specific relationships with acceptable measures of fit (r2 = 94%-56%) for peanut and tree nut sIgE testing at the extract and molecular-level, in order from highest to lowest: Ara h 2, Ara h 6, Jug r 1, Ana o 3, Ara h 1, Jug r 2, and Cor a 9.CONCLUSION: Our models support the notion that quantitative conversion is possible between sIgE multiplex platforms for extracts and molecular allergens and may provide options to aggregate data for future meta-analysis.",
keywords = "Adolescent, Allergens, Antigens, Plant, Arachis, Austria, Canada, Child, Humans, Immunoglobulin E, Nuts, Peanut Hypersensitivity",
author = "Hoang, {Jennifer A} and Alper Celik and Christian Lupinek and Rudolf Valenta and Lucy Duan and Ruixue Dai and Brydges, {May G} and Aim{\'e}e Dubeau and Claire L{\'e}pine and Samantha Wong and Mara Alexanian-Farr and Ahuva Magder and Padmaja Subbarao and Upton, {Julia E M} and Klara Schmidthaler and Zsolt Sz{\'e}pfalusi and Arun Ramani and Thomas Eiwegger",
note = "Funding Information: Dr Lupinek reports personal fees from Thermo Fisher, outside the submitted work. Dr Valenta has received research grants from Viravaxx, Vienna, Austria and serves as a consultant for this company, and received grants from HVD Life‐Sciences, Vienna, Austria, outside the submitted work. Dr Upton reports personal fees from Kaleo, personal fees from ALK, personal fees from Bausch Health, is a member of the advisory board of Emerade, and is a sub‐investigator on clinical trials of DBV Therapeutics, Aimmune, Regeneron, and CIHR, outside the submitted work; and is Section Chair of Food Allergy and Anaphylaxis, Canadian Society of Allergy and Clinical Immunology and is a member of the Healthcare Advisory Board, Food Allergy Canada. Dr Eiwegger is a site PI of a DBV sponsored global study, reports grants from Innovation Fund Denmark, CIHR, is a site CO‐I of a Regeneron sponsored study, outside the submitted work. He is the Co‐I or scientific lead in three investigator‐initiated oral immunotherapy trials supported by the Allergy and Anaphylaxis Program at SickKids, outside of the submitted work. He reports personal fees from ALK and is on a local and international advisory board of ALK. Outside of this project, Dr Eiwegger collaborates with MADX in a government‐funded project. Dr Eiwegger serves as associate editor for Allergy and is in the editorial board of the IAA and the review board of JACI. The other authors have nothing to disclose. Funding Information: This work was supported by funds from the Hospital for Sick Children HSBC Bank Canada Catalyst Research Grant, the Innovation Fund Denmark Grant (no. 6159‐00005A), and the Ontario Lung Association. Publisher Copyright: {\textcopyright} 2020 EAACI and John Wiley and Sons A/S. Published by John Wiley and Sons Ltd.",
year = "2021",
month = mar,
doi = "10.1111/all.14529",
language = "English",
volume = "76",
pages = "831--841",
journal = "Allergy: European Journal of Allergy and Clinical Immunology",
issn = "0105-4538",
publisher = "Wiley-Blackwell Publishing Ltd",
number = "3",
}