@inbook{ef408ce2a41b4b1ca5cd326e7222d541,
title = "Methods to study tumor surveillance using tumor cell transplantation into genetically engineered mice",
abstract = "When a tumor evolves, there is constant crosstalk between the transformed cells and cells of the immune system. Transplantation of well-established tumor cell lines into genetically engineered mice is a valuable tool to study the contribution of a gene of interest to tumor surveillance. These methods bear several advantages: first, such cell lines are well characterized; second, much data for reference exist; and third, the impact of the immune system can be separated from tumor cell intrinsic effects. Here, we provide protocols for tumor cell transplantations to address the role of a specific gene product in tumor surveillance. We furthermore describe several approaches to define the impact of natural killer cells and T cells, such as cell depletion and adoptive transfer experiments or use of different genetically modified mice.",
keywords = "B16F10, EG7, EL4, MC38, Natural killer cells, RMA-S, T cells, Tumor immune surveillance, Immunologic Surveillance, Cell Transplantation/methods, Neoplasm Transplantation/immunology, Mice, Transgenic, Adoptive Transfer, Biomarkers/metabolism, Gene Knockout Techniques, Neoplasms/genetics, Animals, Bone Marrow/metabolism, Cell Line, Tumor, Mice",
author = "Eva Bauer and Agnieszka Witalisz and Birgit Strobl and Dagmar Stoiber",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2015. All rights are reserved.",
year = "2015",
month = jan,
day = "30",
doi = "10.1007/978-1-4939-2297-0_22",
language = "English",
isbn = "9781493922963",
volume = "1267",
series = "Methods in molecular biology (Clifton, N.J.)",
publisher = "Springer New York",
pages = "439--456",
booktitle = "Mouse Models of Cancer",
address = "United States",
}