Methods to study tumor surveillance using tumor cell transplantation into genetically engineered mice

Eva Bauer, Agnieszka Witalisz, Birgit Strobl, Dagmar Stoiber

Research output: Contribution to book/report/conference proceedingChapter in book/report

Abstract

When a tumor evolves, there is constant crosstalk between the transformed cells and cells of the immune system. Transplantation of well-established tumor cell lines into genetically engineered mice is a valuable tool to study the contribution of a gene of interest to tumor surveillance. These methods bear several advantages: first, such cell lines are well characterized; second, much data for reference exist; and third, the impact of the immune system can be separated from tumor cell intrinsic effects. Here, we provide protocols for tumor cell transplantations to address the role of a specific gene product in tumor surveillance. We furthermore describe several approaches to define the impact of natural killer cells and T cells, such as cell depletion and adoptive transfer experiments or use of different genetically modified mice.

Original languageEnglish
Title of host publicationMouse Models of Cancer
Subtitle of host publicationMethods and Protocols
PublisherSpringer New York
Pages439-456
Number of pages18
Volume1267
ISBN (Electronic)9781493922970
ISBN (Print)9781493922963
DOIs
Publication statusPublished - 30 Jan 2015
Externally publishedYes

Publication series

NameMethods in molecular biology (Clifton, N.J.)
ISSN (Print)1064-3745

Keywords

  • B16F10
  • EG7
  • EL4
  • MC38
  • Natural killer cells
  • RMA-S
  • T cells
  • Tumor immune surveillance
  • Immunologic Surveillance
  • Cell Transplantation/methods
  • Neoplasm Transplantation/immunology
  • Mice, Transgenic
  • Adoptive Transfer
  • Biomarkers/metabolism
  • Gene Knockout Techniques
  • Neoplasms/genetics
  • Animals
  • Bone Marrow/metabolism
  • Cell Line, Tumor
  • Mice

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (all)
  • Medicine (all)

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