Jug r 6 is the allergenic vicilin present in walnut responsible for IgE cross-reactivities to other tree nuts and seeds

Pawel Dubiela, Stefan Kabasser, Nicolas Smargiasso, Sabine Geiselhart, Merima Bublin, Christine Hafner, Gabriel Mazzucchelli, Karin Hoffmann-Sommergruber

Research output: Journal article (peer-reviewed)Journal article

16 Citations (Scopus)

Abstract

Walnuts are ranked high in the list of the culprit foods inducing severe allergic reactions. Jug r 2 has been identified as a major allergen in common walnut by cDNA cloning from a somatic cell line. So far, studies were performed on the allergenic activity of recombinant Jug r 2, yet there is still no evidence about the physicochemical characteristics of the natural allergen. Therefore, we aimed to purify and deeply characterize natural Jug r 2 and to assess IgE cross-reactivity among vicilins from different tree nuts. Extensive mass spectrometry analysis of the obtained purified vicilin allowed identification of the protein sequence that displayed only 44% identity to Jug r 2. The newly identified vicilin (Jug r 6) was recognized by IgE of 26% in walnut allergic patients' sera tested. In contrast to Jug r 2, Jug r 6 displayed a remarkable level of cross-reactivity when tested with homologues from hazelnut, sesame and pistachio. It is the first report showing the necessity of proteomic studies to improve allergy component resolved diagnosis.

Original languageEnglish
Article number11366
Pages (from-to)11366
JournalScientific Reports
Volume8
Issue number1
DOIs
Publication statusPublished - 1 Dec 2018

Keywords

  • Adult
  • Allergens/immunology
  • Amino Acid Sequence
  • Cross Reactions
  • Female
  • Humans
  • Immunoglobulin E/blood
  • Juglans/chemistry
  • Male
  • Nuts/immunology
  • Protein Stability
  • Proteolysis
  • Seed Storage Proteins/chemistry
  • Seeds/immunology
  • Temperature
  • Young Adult

Fingerprint

Dive into the research topics of 'Jug r 6 is the allergenic vicilin present in walnut responsible for IgE cross-reactivities to other tree nuts and seeds'. Together they form a unique fingerprint.

Cite this