IgE recognition of the house dust mite allergen Der p 37 is associated with asthma

Huey-Jy Huang, Yvonne Resch-Marat, Anne Casset, Margit Weghofer, Petra Zieglmayer, René Zieglmayer, Patrick Lemell, Friedrich Horak, Kuan-Wei Chen, Ekaterina Potapova, Paolo M Matricardi, Gabrielle Pauli, Monika Grote, Rudolf Valenta, Susanne Vrtala

Research output: Journal article (peer-reviewed)Journal article

19 Citations (Scopus)


BACKGROUND: House dust mite (HDM) allergens are major elicitors of allergic reactions worldwide.

OBJECTIVE: Identification, characterization, and evaluation of diagnostic utility of a new important HDM allergen was performed.

METHODS: A cDNA coding for a new Dermatophagoides pteronyssinus (Dp) allergen, Der p 37, was isolated from a Dp expression library with allergic patients' IgE antibodies. Recombinant Der p 37 (rDer p 37) expressed in Escherichia coli was purified, then characterized by mass spectrometry, circular dichroism, and IgE reactivity by ImmunoCAP ISAC technology with sera from 111 clinically defined HDM-allergic patients. The allergenic activity of rDer p 37 was studied by basophil activation and CD4+ T-cell responses by carboxyfluorescein diacetate succinimidyl ester dilution assays. Specific antibodies raised against rDer p 37 were used for the ultrastructural localization of Der p 37 in mites by immunogold transmission electron microscopy.

RESULTS: Der p 37, a 26 kDa allergen with homology to chitin-binding proteins, is immunologically distinct from Der p 15, 18, and 23. It is located in the peritrophic membrane of fecal pellets. Der p 37 reacted with IgE antibodies from a third of HDM-allergic patients and induced specific basophil- and CD4+ T-cell activation. Der p 37 IgE-positive patients had significantly higher IgE levels to major HDM allergens, reacted with more HDM allergens, and had a higher risk (odds ratio = 3.1) of asthma compared to Der p 37-negative patients.

CONCLUSIONS: Der p 37, a new Dp allergen recognized by a third of HDM-allergic patients, may serve as a surrogate marker for severe HDM sensitization and asthma.

Original languageEnglish
Pages (from-to)1031-1043
Number of pages13
JournalJournal of Allergy and Clinical Immunology
Issue number3
Publication statusPublished - Mar 2022


  • Allergens
  • Animals
  • Antigens, Dermatophagoides
  • Arthropod Proteins
  • Asthma/diagnosis
  • Dust
  • Escherichia coli/genetics
  • Humans
  • Hypersensitivity
  • Immunoglobulin E
  • Pyroglyphidae


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