Hydrolysis of 4-methylumbelliferyl-β-D-glucuronide in differing sample fractions of river waters and its implication for the detection of fecal pollution

A. H. Farnleitner, L. Hocke, C. Beiwl, G. G. Kavka, R. L. Mach

Research output: Journal article (peer-reviewed)Journal article

33 Citations (Scopus)

Abstract

The hydrolysis rate of 4-methylumbelliferyl-β-D-glucuronide (MUG-HR) was determined in unamended samples, filtered samples, and in corresponding buffer resuspended filter residues of various river waters of slight to excessive fecal pollution covering a four orders of magnitude range. Regression analysis of the log MUG-HR of the unamended water samples versus the log MUG-HR of the filter residues revealed a highly significant linear relationship (R2=0.94; p<0.001). The median of the MUG-HR of the filtrated water samples was about 10% the MUG-HR of the corresponding unamended water samples. If MUG-HR determinations were used as a surrogate for estimating fecal coliform contamination, both the MUG-HR of the unamended water samples and the MUG-HR of the filter residues would have been equally adequate techniques at river sites of higher fecal pollution levels. However, at river locations of decreased fecal pollution, MUG-HR determination of filter residues appeared to be the more sensitive technique in order to estimate fecal coliform concentrations.

Original languageEnglish
Pages (from-to)975-981
Number of pages7
JournalWater Research
Volume36
Issue number4
DOIs
Publication statusPublished - 2002
Externally publishedYes

Keywords

  • 4-methylumbelliferyl-β-D-glucuronide hydrolysis
  • Fecal pollution
  • Monitoring
  • Rivers
  • Environmental Monitoring/methods
  • Reproducibility of Results
  • Hymecromone/analogs & derivatives
  • Hydrolysis
  • Specimen Handling
  • Water Pollutants/analysis
  • Fluorescent Dyes/analysis
  • Feces/microbiology
  • Sensitivity and Specificity
  • Enterobacteriaceae

ASJC Scopus subject areas

  • Water Science and Technology
  • Ecological Modeling
  • Pollution
  • Waste Management and Disposal
  • Environmental Engineering
  • Civil and Structural Engineering

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