Findings from an outbreak of carbapenem-resistant Klebsiella pneumoniae emphasize the role of antibiotic treatment for cross transmission

Stefan Borgmann, Yvonne Pfeifer, Laura Becker, Beate Rieß, Rabea Siegmund, Ulrich Sagel

Research output: Journal article (peer-reviewed)Journal article

11 Citations (Scopus)

Abstract

PURPOSE: In January 2015, we noticed by rectal swab analyses that seven of 23 patients at an early rehabilitation ward had been colonized with carbapenem-resistant Klebsiella pneumoniae (CKP). Here, we describe risk factors for CKP acquisition.

METHODS: In the present study, the outbreak is described and risk factors for CKP acquisition are examined, e.g., antibiotic treatment. Microbiological analyses including corresponding results were examined to study when colonization with CKP occurred and whether patients had suffered from diarrhea. To examine whether spread of bacteria was clonal, multi-locus sequence typing as well as Xbal macrorestriction and pulsed-field gel electrophoresis was performed. The presence of carbapenmase was examined by PCR analysis. Through univariate analysis of risk factors in the small study sample, the role of antibiotic consumption, isolation procedures, patient's age, gender, and Barthel index on colonization was elucidated.

RESULTS: Clonal spread of the novel sequence type (ST)2255 was identified. Additionally, one patient was colonized with Escherichia coli and Serratia marcescens, both resistant to carbapenems, while a further patient carried another carbapenem-resistant E. coli strain. In all isolates, carbapenemase gene bla OXA-48 was found to be located on a conjugative plasmid (60 kb), suggesting in vivo transmission from CKP to E. coli and S. marcescens. Univariate tests indicated that antibiotic treatment was the only risk factor showing a significant association with being colonized by CKP. In addition, the likelihood of diarrhea appeared to be higher in this group. Antibiotic treatment was associated with CKP colonization, whereas patients´ age, gender, Barthel index at admission, and residence with a CKP-colonized roommate were not. Diarrhea also seemed to support to distribution of CKP.

CONCLUSIONS: In this small outbreak, antibiotic treatment seemed to be the predominant risk factor for monoclonal transmission of bla OXA-48 positive CKP.

Original languageEnglish
Pages (from-to)103-112
Number of pages10
JournalInfection
Volume46
Issue number1
DOIs
Publication statusPublished - 1 Feb 2018

Keywords

  • Adult
  • Aged
  • Aged, 80 and over
  • Carbapenems/pharmacology
  • Cross Infection/epidemiology
  • Disease Outbreaks
  • Drug Resistance, Bacterial
  • Female
  • Germany/epidemiology
  • Humans
  • Klebsiella Infections/epidemiology
  • Klebsiella pneumoniae/physiology
  • Male
  • Microbial Sensitivity Tests
  • Middle Aged
  • Risk Factors
  • beta-Lactamases/analysis

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