TY - JOUR
T1 - Features of the Human Antibody Response against the Respiratory Syncytial Virus Surface Glycoprotein G
AU - Borochova, Kristina
AU - Niespodziana, Katarzyna
AU - Stenberg Hammar, Katarina
AU - van Hage, Marianne
AU - Hedlin, Gunilla
AU - Söderhäll, Cilla
AU - Focke-Tejkl, Margarete
AU - Valenta, Rudolf
N1 - Funding Information:
Funding: This study was funded by grants F4605, by project No. P29398, by the International PhD Program “Inflammation and Immunity” (IAI) of the Austrian Science Fund (FWF) and by Viravaxx, Vienna, Austria. R.V. is a recipient of a Megagrant of the Government of the Russian Federation, grant No 14. W03.31.0024. Furthermore, this study was funded by the Swedish Heart-Lung Foundation, the Centre for Allergy Research at Karolinska Institutet, the Swedish Association for Allergology (SFFA), Freemason Child House Foundation in Stockholm, Konsul Th C Berghs stiftelse, the Swedish Asthma and Allergy Association’s Research Foundation, the Swedish Research Council, the Swedish Cancer and Allergy Foundation and Region Stockholm (ALF-project).
Funding Information:
This study was funded by grants F4605, by project No. P29398, by the International PhD Program ?Inflammation and Immunity? (IAI) of the Austrian Science Fund (FWF) and by Viravaxx, Vienna, Austria. R.V. is a recipient of a Megagrant of the Government of the Russian Federation, grant No 14. W03.31.0024. Furthermore, this study was funded by the Swedish Heart-Lung Foundation, the Centre for Allergy Research at Karolinska Institutet, the Swedish Association for Allergology (SFFA), Freemason Child House Foundation in Stockholm, Konsul Th C Berghs stiftelse, the Swedish Asthma and Allergy Association?s Research Foundation, the Swedish Research Council, the Swedish Cancer and Allergy Foundation and Region Stockholm (ALF-project).
Publisher Copyright:
© 2020 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2020/6/25
Y1 - 2020/6/25
N2 - Respiratory syncytial virus (RSV) infections are a major cause of serious respiratory disease in infants. RSV occurs as two major subgroups A and B, which mainly differ regarding the surface glycoprotein G. The G protein is important for virus attachment and G-specific antibodies can protect against infection. We expressed the surface-exposed part of A2 strain-derived G (A2-G) in baculovirus-infected insect cells and synthesized overlapping peptides spanning complete A2-G. The investigation of the natural IgG response of adult subjects during a period of one year showed that IgG antibodies (i) recognize G significantly stronger than the fusion protein F0, (ii) target mainly non-conformational, sequential peptide epitopes from the exposed conserved region but also buried peptides, and (iii) exhibit a scattered but constant recognition profile during the observation period. The IgG subclass reactivity profile (IgG1 > IgG2 > IgG4 = IgG3) was indicative of a mixed Th1/Th2 response. Two strongly RSV-neutralizing sera including the 1st WHO standard contained high IgG anti-G levels. G-specific IgG increased strongly in children after wheezing attacks suggesting RSV as trigger factor. Our study shows that RSV G and G-derived peptides are useful for serological diagnosis of RSV-triggered exacerbations of respiratory diseases and underlines the importance of G for development of RSV-neutralizing vaccines.
AB - Respiratory syncytial virus (RSV) infections are a major cause of serious respiratory disease in infants. RSV occurs as two major subgroups A and B, which mainly differ regarding the surface glycoprotein G. The G protein is important for virus attachment and G-specific antibodies can protect against infection. We expressed the surface-exposed part of A2 strain-derived G (A2-G) in baculovirus-infected insect cells and synthesized overlapping peptides spanning complete A2-G. The investigation of the natural IgG response of adult subjects during a period of one year showed that IgG antibodies (i) recognize G significantly stronger than the fusion protein F0, (ii) target mainly non-conformational, sequential peptide epitopes from the exposed conserved region but also buried peptides, and (iii) exhibit a scattered but constant recognition profile during the observation period. The IgG subclass reactivity profile (IgG1 > IgG2 > IgG4 = IgG3) was indicative of a mixed Th1/Th2 response. Two strongly RSV-neutralizing sera including the 1st WHO standard contained high IgG anti-G levels. G-specific IgG increased strongly in children after wheezing attacks suggesting RSV as trigger factor. Our study shows that RSV G and G-derived peptides are useful for serological diagnosis of RSV-triggered exacerbations of respiratory diseases and underlines the importance of G for development of RSV-neutralizing vaccines.
UR - http://www.scopus.com/inward/record.url?scp=85086921049&partnerID=8YFLogxK
U2 - 10.3390/vaccines8020337
DO - 10.3390/vaccines8020337
M3 - Journal article
C2 - 32630611
SN - 2076-393X
VL - 8
SP - 1
EP - 21
JO - Vaccines
JF - Vaccines
IS - 2
M1 - 337
ER -