Exploring the use of hydrophilic ionic liquids for the lysis of fungal structures

Johanna Kreuter; Christian Zimmermann; Katharina Bica-Schröder; Dorottya Mikoczi; Matthias Steiger; Astrid R Mach-Aigner; Robert L Mach; Andreas H Farnleitner; Georg H Reischer

doi:10.1016/j.mimet.2026.107515

Exploring the use of hydrophilic ionic liquids for the lysis of fungal structures

Johanna Kreuter
, Christian Zimmermann
, Katharina Bica-Schröder
, Dorottya Mikoczi
, Matthias Steiger
, Astrid R Mach-Aigner
, Robert L Mach
, Andreas H Farnleitner
, Georg H Reischer^*

^*Corresponding author for this work

Department of Water Quality and Health

Research output: Journal article (peer-reviewed) › Journal article

Abstract

This study explores the use of hydrophilic ionic liquids for lysis of Aspergillus niger and Trichoderma reesei conidia and mycelium to provide DNA suitable for PCR-based assays, demonstrating their potential as simple, cost-effective, and gentle lysis agents and potentially enabling rapid DNA extraction for diverse molecular applications.

Original language	English
Article number	107515
Pages (from-to)	107515
Journal	Journal of Microbiological Methods
Volume	245
Early online date	15 Apr 2026
DOIs	https://doi.org/10.1016/j.mimet.2026.107515
Publication status	E-pub ahead of print - 15 Apr 2026

Access to Document

10.1016/j.mimet.2026.107515

Cite this

Kreuter, J., Zimmermann, C., Bica-Schröder, K., Mikoczi, D., Steiger, M., Mach-Aigner, A. R., Mach, R. L., Farnleitner, A. H., & Reischer, G. H. (2026). Exploring the use of hydrophilic ionic liquids for the lysis of fungal structures. Journal of Microbiological Methods, 245, 107515. Article 107515. Advance online publication. https://doi.org/10.1016/j.mimet.2026.107515

@article{4d5749be5ae543c1b47adfd83109f7cd,

title = "Exploring the use of hydrophilic ionic liquids for the lysis of fungal structures",

abstract = "This study explores the use of hydrophilic ionic liquids for lysis of Aspergillus niger and Trichoderma reesei conidia and mycelium to provide DNA suitable for PCR-based assays, demonstrating their potential as simple, cost-effective, and gentle lysis agents and potentially enabling rapid DNA extraction for diverse molecular applications.",

author = "Johanna Kreuter and Christian Zimmermann and Katharina Bica-Schr{\"o}der and Dorottya Mikoczi and Matthias Steiger and Mach-Aigner, \{Astrid R\} and Mach, \{Robert L\} and Farnleitner, \{Andreas H\} and Reischer, \{Georg H\}",

note = "Publisher Copyright: {\textcopyright} 2024",

year = "2026",

month = apr,

day = "15",

doi = "10.1016/j.mimet.2026.107515",

language = "English",

volume = "245",

pages = "107515",

journal = "Journal of Microbiological Methods",

issn = "0167-7012",

publisher = "Elsevier B.V.",

}

TY - JOUR

T1 - Exploring the use of hydrophilic ionic liquids for the lysis of fungal structures

AU - Kreuter, Johanna

AU - Zimmermann, Christian

AU - Bica-Schröder, Katharina

AU - Mikoczi, Dorottya

AU - Steiger, Matthias

AU - Mach-Aigner, Astrid R

AU - Mach, Robert L

AU - Farnleitner, Andreas H

AU - Reischer, Georg H

PY - 2026/4/15

Y1 - 2026/4/15

N2 - This study explores the use of hydrophilic ionic liquids for lysis of Aspergillus niger and Trichoderma reesei conidia and mycelium to provide DNA suitable for PCR-based assays, demonstrating their potential as simple, cost-effective, and gentle lysis agents and potentially enabling rapid DNA extraction for diverse molecular applications.

AB - This study explores the use of hydrophilic ionic liquids for lysis of Aspergillus niger and Trichoderma reesei conidia and mycelium to provide DNA suitable for PCR-based assays, demonstrating their potential as simple, cost-effective, and gentle lysis agents and potentially enabling rapid DNA extraction for diverse molecular applications.

UR - https://www.scopus.com/pages/publications/105036737903

U2 - 10.1016/j.mimet.2026.107515

DO - 10.1016/j.mimet.2026.107515

M3 - Journal article

C2 - 41997303

SN - 0167-7012

VL - 245

SP - 107515

JO - Journal of Microbiological Methods

JF - Journal of Microbiological Methods

M1 - 107515

ER -

Exploring the use of hydrophilic ionic liquids for the lysis of fungal structures

Abstract

Access to Document

Other files and links

Fingerprint

Cite this