Evaluation of genotoxic pressure along the sava river

Stoimir Kolarević, Mustafa Aborgiba, Margareta Kračun-Kolarević, Jovana Kostić, Predrag Simonović, Vladica Simić, Aleksandra Milošković, Georg Reischer, Andreas Farnleitner, Zoran Gačić, Radmila Milačič, Tea Zuliani, Janja Vidmar, Marija Pergal, Marina Piria, Momir Paunović, Branka Vuković-Gačić

Research output: Journal article (peer-reviewed)Journal article

23 Citations (Scopus)


In this study we have performed a comprehensive genotoxicological survey along the 900 rkm of the Sava River. In total, 12 sites were chosen in compliance with the goals of GLOBAQUA project dealing with the effects of multiple stressors on biodiversity and functioning of aquatic ecosystems. The genotoxic potential was assessed using a complex battery of bioassays performed in prokaryotes and aquatic eukaryotes (freshwater fish). Battery comprised evaluation of mutagenicity by SOS/umuC test in Salmonella typhimuriumTA1535/ pSK1002. The level of DNA damage as a biomarker of exposure (comet assay) and biomarker of effect (micronucleus assay) and the level of oxidative stress as well (Fpg-modified comet assay) was studied in blood cells of bleak and spirlin (Alburnus alburnus/ Alburnoides bipunctatus respectively). Result indicated differential sensitivity of applied bioassays in detection of genotoxic pressure. The standard and Fpg-modified comet assay showed higher potential in differentiation of the sites based on genotoxic potential in comparison with micronucleus assay and SOS/umuC test. Our data represent snapshot of the current status of the river which indicates the presence of genotoxic potential along the river which can be traced to the deterioration of quality of the Sava River by communal and industrialwastewaters. The major highlight of the study is that we have provided complex set of data obtained from a single source (homogeneity of analyses for all samples).

Original languageEnglish
Article numbere0162450
JournalPLoS ONE
Issue number9
Publication statusPublished - Sept 2016
Externally publishedYes

ASJC Scopus subject areas

  • Multidisciplinary


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