TY - JOUR
T1 - Dynamics of Vibrio cholerae abundance in Austrian saline lakes, assessed with quantitative solid-phase cytometry
AU - Schauer, Sonja
AU - Jakwerth, Stefan
AU - Bliem, Rupert
AU - Baudart, Julia
AU - Lebaron, Philippe
AU - Huhulescu, Steliana
AU - Kundi, Michael
AU - Herzig, Alois
AU - Farnleitner, Andreas H.
AU - Sommer, Regina
AU - Kirschner, Alexander
N1 - Publisher Copyright:
© 2015 Society for Applied Microbiology and John Wiley & Sons Ltd.
PY - 2015/11/1
Y1 - 2015/11/1
N2 - In order to elucidate the main predictors of Vibrio cholerae dynamics and to estimate the risk of Vibrio cholera-related diseases, a recently developed direct detection approach based on fluorescence in situ hybridization and solid-phase cytometry (CARD-FISH/SPC) was applied in comparison to cultivation for water samples from the lake Neusiedler See, Austria and three shallow alkaline lakes over a period of 20 months. Vibrio cholerae attached to crustacean zooplankton was quantified via FISH and epifluorescence microscopy. Concentrations obtained by CARD-FISH/SPC were significantly higher than those obtained by culture in 2011, but were mostly of similar magnitude in 2012. Maximum cell numbers were 1.26×106 V. cholerae per L in Neusiedler See and 7.59×107 V. cholerae per L in the shallow alkaline lakes. Only on a few occasions during summer was the crustacean zooplankton the preferred habitat for V. cholerae. In winter, V. cholerae was not culturable but could be quantified at all sites with CARD-FISH/SPC. Beside temperature, suspended solids, zooplankton and ammonium were the main predictors of V. cholerae abundance in Neusiedler See, while in the shallow alkaline lakes it was organic carbon, conductivity and phosphorus. Based on the obtained concentrations a first estimation of the health risk for visitors of the lake could be performed.
AB - In order to elucidate the main predictors of Vibrio cholerae dynamics and to estimate the risk of Vibrio cholera-related diseases, a recently developed direct detection approach based on fluorescence in situ hybridization and solid-phase cytometry (CARD-FISH/SPC) was applied in comparison to cultivation for water samples from the lake Neusiedler See, Austria and three shallow alkaline lakes over a period of 20 months. Vibrio cholerae attached to crustacean zooplankton was quantified via FISH and epifluorescence microscopy. Concentrations obtained by CARD-FISH/SPC were significantly higher than those obtained by culture in 2011, but were mostly of similar magnitude in 2012. Maximum cell numbers were 1.26×106 V. cholerae per L in Neusiedler See and 7.59×107 V. cholerae per L in the shallow alkaline lakes. Only on a few occasions during summer was the crustacean zooplankton the preferred habitat for V. cholerae. In winter, V. cholerae was not culturable but could be quantified at all sites with CARD-FISH/SPC. Beside temperature, suspended solids, zooplankton and ammonium were the main predictors of V. cholerae abundance in Neusiedler See, while in the shallow alkaline lakes it was organic carbon, conductivity and phosphorus. Based on the obtained concentrations a first estimation of the health risk for visitors of the lake could be performed.
UR - http://www.scopus.com/inward/record.url?scp=84949318096&partnerID=8YFLogxK
U2 - 10.1111/1462-2920.12861
DO - 10.1111/1462-2920.12861
M3 - Journal article
C2 - 25847810
AN - SCOPUS:84949318096
SN - 1462-2912
VL - 17
SP - 4366
EP - 4378
JO - Environmental Microbiology
JF - Environmental Microbiology
IS - 11
ER -