Dissecting the Sensitization Profiles to Parvalbumins From 12 Freshwater Fish Species to Improve Diagnosis of Fish Allergy

T Kalic Kamath; T Djukic; S D Kamath; N Lengger; S Ottersbach; K Uranowska-Kostrubala; M Mladenovic Stokanic; K H Park; P Forstenlechner; M Aumayr; G Lamorte; T Velickovic Cirkovic; W Hemmer; H Breiteneder; C Hafner

doi:10.18176/jiaci.1069

Dissecting the Sensitization Profiles to Parvalbumins From 12 Freshwater Fish Species to Improve Diagnosis of Fish Allergy

T Kalic Kamath, T Djukic, S D Kamath, N Lengger, S Ottersbach, K Uranowska-Kostrubala, M Mladenovic Stokanic, K H Park, P Forstenlechner, M Aumayr, G Lamorte, T Velickovic Cirkovic, W Hemmer, H Breiteneder, C Hafner

Research output: Journal article (peer-reviewed) › Journal article

Abstract

BACKGROUND AND OBJECTIVES: Many fish-allergic patients only react to certain fish species and may tolerate others, mostly because of IgE-mediated recognition of specific epitopes on the major allergen parvalbumin. However, the considerable number of fish species consumed makes it challenging to identify which species are allergenic and which are tolerated by individual patients. In order to improve the diagnosis of fish allergy, we investigated IgE-mediated reactivity to parvalbumins from 12 freshwater fish species that are largely underrepresented in diagnostic tests.

METHODS: Parvalbumins were purified from 12 freshwater fish species belonging to 8 families, and their isoform composition was analyzed using mass spectrometry. IgE specific for each parvalbumin was quantified in serum samples from 66 fish allergic individuals, and basophil activation tests were performed for 5 patients. Crosswise inhibition assays were carried out for all parvalbumins for 7 patients to investigate cross-reactivity between the parvalbumins from the different species.

RESULTS: IgE binding and cross-linking potency of the parvalbumins differed, with the strongest reactivities observed for 4 parvalbumins from the salmonid family (results positive for 89%-95% of patients) and the weakest for parvalbumins from Wels catfish, European eel, and tench (results negative for ≥50% of patients). Ninety percent of the patients with negative results for Wels catfish parvalbumin also had negative results for additional parvalbumins from multiple species. Inhibition assays revealed variable recognition of epitopes by several patients, with the primary sensitizers most frequently being parvalbumins from salmonids and percids.

CONCLUSION: Including freshwater salmonids in the diagnostic work-up for fish allergy may help to identify most fish-allergic patients. IgE to Wels catfish could help distinguish between polysensitized and oligosensitized patients.

Original language	English
Pages (from-to)	0
Journal	Journal of Investigational Allergology and Clinical Immunology
DOIs	https://doi.org/10.18176/jiaci.1069
Publication status	E-pub ahead of print - 14 Mar 2025

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10.18176/jiaci.1069

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Kalic Kamath, T., Djukic, T., Kamath, S. D., Lengger, N., Ottersbach, S., Uranowska-Kostrubala, K., Mladenovic Stokanic, M., Park, K. H., Forstenlechner, P., Aumayr, M., Lamorte, G., Velickovic Cirkovic, T., Hemmer, W., Breiteneder, H., & Hafner, C. (2025). Dissecting the Sensitization Profiles to Parvalbumins From 12 Freshwater Fish Species to Improve Diagnosis of Fish Allergy. Journal of Investigational Allergology and Clinical Immunology, 0. Advance online publication. https://doi.org/10.18176/jiaci.1069

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title = "Dissecting the Sensitization Profiles to Parvalbumins From 12 Freshwater Fish Species to Improve Diagnosis of Fish Allergy",

abstract = "BACKGROUND AND OBJECTIVES: Many fish-allergic patients only react to certain fish species and may tolerate others, mostly because of IgE-mediated recognition of specific epitopes on the major allergen parvalbumin. However, the considerable number of fish species consumed makes it challenging to identify which species are allergenic and which are tolerated by individual patients. In order to improve the diagnosis of fish allergy, we investigated IgE-mediated reactivity to parvalbumins from 12 freshwater fish species that are largely underrepresented in diagnostic tests.METHODS: Parvalbumins were purified from 12 freshwater fish species belonging to 8 families, and their isoform composition was analyzed using mass spectrometry. IgE specific for each parvalbumin was quantified in serum samples from 66 fish allergic individuals, and basophil activation tests were performed for 5 patients. Crosswise inhibition assays were carried out for all parvalbumins for 7 patients to investigate cross-reactivity between the parvalbumins from the different species.RESULTS: IgE binding and cross-linking potency of the parvalbumins differed, with the strongest reactivities observed for 4 parvalbumins from the salmonid family (results positive for 89%-95% of patients) and the weakest for parvalbumins from Wels catfish, European eel, and tench (results negative for ≥50% of patients). Ninety percent of the patients with negative results for Wels catfish parvalbumin also had negative results for additional parvalbumins from multiple species. Inhibition assays revealed variable recognition of epitopes by several patients, with the primary sensitizers most frequently being parvalbumins from salmonids and percids.CONCLUSION: Including freshwater salmonids in the diagnostic work-up for fish allergy may help to identify most fish-allergic patients. IgE to Wels catfish could help distinguish between polysensitized and oligosensitized patients.",

author = "{Kalic Kamath}, T and T Djukic and Kamath, {S D} and N Lengger and S Ottersbach and K Uranowska-Kostrubala and {Mladenovic Stokanic}, M and Park, {K H} and P Forstenlechner and M Aumayr and G Lamorte and {Velickovic Cirkovic}, T and W Hemmer and H Breiteneder and C Hafner",

year = "2025",

month = mar,

day = "14",

doi = "10.18176/jiaci.1069",

language = "English",

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journal = "Journal of Investigational Allergology and Clinical Immunology",

issn = "1018-9068",

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}

Kalic Kamath, T, Djukic, T, Kamath, SD, Lengger, N, Ottersbach, S, Uranowska-Kostrubala, K, Mladenovic Stokanic, M, Park, KH, Forstenlechner, P, Aumayr, M, Lamorte, G, Velickovic Cirkovic, T, Hemmer, W, Breiteneder, H & Hafner, C 2025, 'Dissecting the Sensitization Profiles to Parvalbumins From 12 Freshwater Fish Species to Improve Diagnosis of Fish Allergy', Journal of Investigational Allergology and Clinical Immunology, pp. 0. https://doi.org/10.18176/jiaci.1069

TY - JOUR

T1 - Dissecting the Sensitization Profiles to Parvalbumins From 12 Freshwater Fish Species to Improve Diagnosis of Fish Allergy

AU - Kalic Kamath, T

AU - Djukic, T

AU - Kamath, S D

AU - Lengger, N

AU - Ottersbach, S

AU - Uranowska-Kostrubala, K

AU - Mladenovic Stokanic, M

AU - Park, K H

AU - Forstenlechner, P

AU - Aumayr, M

AU - Lamorte, G

AU - Velickovic Cirkovic, T

AU - Hemmer, W

AU - Breiteneder, H

AU - Hafner, C

PY - 2025/3/14

Y1 - 2025/3/14

N2 - BACKGROUND AND OBJECTIVES: Many fish-allergic patients only react to certain fish species and may tolerate others, mostly because of IgE-mediated recognition of specific epitopes on the major allergen parvalbumin. However, the considerable number of fish species consumed makes it challenging to identify which species are allergenic and which are tolerated by individual patients. In order to improve the diagnosis of fish allergy, we investigated IgE-mediated reactivity to parvalbumins from 12 freshwater fish species that are largely underrepresented in diagnostic tests.METHODS: Parvalbumins were purified from 12 freshwater fish species belonging to 8 families, and their isoform composition was analyzed using mass spectrometry. IgE specific for each parvalbumin was quantified in serum samples from 66 fish allergic individuals, and basophil activation tests were performed for 5 patients. Crosswise inhibition assays were carried out for all parvalbumins for 7 patients to investigate cross-reactivity between the parvalbumins from the different species.RESULTS: IgE binding and cross-linking potency of the parvalbumins differed, with the strongest reactivities observed for 4 parvalbumins from the salmonid family (results positive for 89%-95% of patients) and the weakest for parvalbumins from Wels catfish, European eel, and tench (results negative for ≥50% of patients). Ninety percent of the patients with negative results for Wels catfish parvalbumin also had negative results for additional parvalbumins from multiple species. Inhibition assays revealed variable recognition of epitopes by several patients, with the primary sensitizers most frequently being parvalbumins from salmonids and percids.CONCLUSION: Including freshwater salmonids in the diagnostic work-up for fish allergy may help to identify most fish-allergic patients. IgE to Wels catfish could help distinguish between polysensitized and oligosensitized patients.

AB - BACKGROUND AND OBJECTIVES: Many fish-allergic patients only react to certain fish species and may tolerate others, mostly because of IgE-mediated recognition of specific epitopes on the major allergen parvalbumin. However, the considerable number of fish species consumed makes it challenging to identify which species are allergenic and which are tolerated by individual patients. In order to improve the diagnosis of fish allergy, we investigated IgE-mediated reactivity to parvalbumins from 12 freshwater fish species that are largely underrepresented in diagnostic tests.METHODS: Parvalbumins were purified from 12 freshwater fish species belonging to 8 families, and their isoform composition was analyzed using mass spectrometry. IgE specific for each parvalbumin was quantified in serum samples from 66 fish allergic individuals, and basophil activation tests were performed for 5 patients. Crosswise inhibition assays were carried out for all parvalbumins for 7 patients to investigate cross-reactivity between the parvalbumins from the different species.RESULTS: IgE binding and cross-linking potency of the parvalbumins differed, with the strongest reactivities observed for 4 parvalbumins from the salmonid family (results positive for 89%-95% of patients) and the weakest for parvalbumins from Wels catfish, European eel, and tench (results negative for ≥50% of patients). Ninety percent of the patients with negative results for Wels catfish parvalbumin also had negative results for additional parvalbumins from multiple species. Inhibition assays revealed variable recognition of epitopes by several patients, with the primary sensitizers most frequently being parvalbumins from salmonids and percids.CONCLUSION: Including freshwater salmonids in the diagnostic work-up for fish allergy may help to identify most fish-allergic patients. IgE to Wels catfish could help distinguish between polysensitized and oligosensitized patients.

U2 - 10.18176/jiaci.1069

DO - 10.18176/jiaci.1069

M3 - Journal article

C2 - 40084846

SN - 1018-9068

SP - 0

JO - Journal of Investigational Allergology and Clinical Immunology

JF - Journal of Investigational Allergology and Clinical Immunology

ER -

Dissecting the Sensitization Profiles to Parvalbumins From 12 Freshwater Fish Species to Improve Diagnosis of Fish Allergy

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