Detection of the food allergen celery via loop-mediated isothermal amplification technique

Celine Zahradnik, Roland Martzy, Robert L. Mach, Rudolf Krska, Andreas H. Farnleitner, Kurt Brunner*

*Corresponding author for this work

Research output: Journal article (peer-reviewed)Journal article

17 Citations (Scopus)

Abstract

Since 2005, celery and celery products have to be labeled according to Directive 2003/89/EC due to their allergenic potential. In order to provide a DNA-based, rapid and simple detection method suitable for high-throughput analysis, a loop-mediated isothermal amplification (LAMP) assay for the detection of celery (Apium graveolens) was developed. The assay was tested for specificity for celery since closely related species also hold food relevance. The limit of detection (LOD) for spiked food samples was found to be as low as 7.8 mg of dry celery powder per kilogram. An evaluation of different amplification and detection platforms was performed to show reliable detection independent from the instrument used for amplification (thermal cycler or heating block) and detection mechanisms (real-time fluorescence detection, agarose gel electrophoresis or nucleic acid staining). The analysis of 10 commercial food samples representing diverse and complex food matrices, and a false-negative rate of 0 % for approximately 24 target copies or 0.08 ng celery DNA for three selected food matrices show that LAMP has the potential to be used as an alternative strategy for the detection of allergenic celery. The performance of the developed LAMP assay turned out to be equal or superior to the best available PCR assay for the detection of celery in food products.

Original languageEnglish
Pages (from-to)6827-6833
Number of pages7
JournalAnalytical and Bioanalytical Chemistry
Volume406
Issue number27
DOIs
Publication statusPublished - Nov 2014
Externally publishedYes

Keywords

  • Celery
  • Food allergen
  • Isothermal amplification
  • Loop-mediated isothermal amplification
  • Visual detection

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

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