Conditional ablation of TYK2 in immunity to viral infection and tumor surveillance

Raimund M. Vielnascher; Eva Hainzl; Nicole R. Leitner; Michael Rammerstorfer; David Popp; Agnieszka Witalisz; Rita Rom; Marina Karaghiosoff; Thomas Kolbe; Simone Müller; Thomas Rülicke; Caroline Lassnig; Birgit Strobl; Mathias Müller

doi:10.1007/s11248-014-9795-y

Conditional ablation of TYK2 in immunity to viral infection and tumor surveillance

Raimund M. Vielnascher
, Eva Hainzl
, Nicole R. Leitner
, Michael Rammerstorfer
, David Popp
, Agnieszka Witalisz
, Rita Rom
, Marina Karaghiosoff
, Thomas Kolbe
, Simone Müller
, Thomas Rülicke
, Caroline Lassnig
, Birgit Strobl
, Mathias Müller^*

^*Corresponding author for this work

Research output: Journal article (peer-reviewed) › Journal article

20 Citations (Scopus)

Abstract

Tyrosine kinase 2 (TYK2) has a pivotal role in immunity to infection and tumor surveillance. It is associated with several cytokine receptor chains including type I interferon (IFN) receptor 1 (IFNAR1), interleukin- (IL-) 12 receptor beta 1 (IL-12Rb1) and IL-10R2. We have generated a mouse with a conditional Tyk2 null allele and proved integrity of the conditional Tyk2 locus. TYK2 was successfully removed by the use of ubiquitous and tissue-specific Cre-expressing mouse strains. Myeloid TYK2 was found to critically contribute to the defense against murine cytomegalovirus. Ubiquitous TYK2 ablation severely impaired tumor immunosurveillance, while deletion in myeloid, dendritic or T cells alone showed no effect. The conditional Tyk2 mouse strain will be instrumental to further dissect TYK2 functions in infection, inflammation and cancer.

Original language	English
Pages (from-to)	519-529
Number of pages	11
Journal	Transgenic Research
Volume	23
Issue number	3
DOIs	https://doi.org/10.1007/s11248-014-9795-y
Publication status	Published - Jun 2014
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

Conditional knockout
JAK
Janus kinase
MC38
MCMV
Tumor transplant
Tyrosine kinase 2

ASJC Scopus subject areas

Biotechnology
Animal Science and Zoology
Agronomy and Crop Science
Genetics

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10.1007/s11248-014-9795-y

Cite this

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title = "Conditional ablation of TYK2 in immunity to viral infection and tumor surveillance",

abstract = "Tyrosine kinase 2 (TYK2) has a pivotal role in immunity to infection and tumor surveillance. It is associated with several cytokine receptor chains including type I interferon (IFN) receptor 1 (IFNAR1), interleukin- (IL-) 12 receptor beta 1 (IL-12Rb1) and IL-10R2. We have generated a mouse with a conditional Tyk2 null allele and proved integrity of the conditional Tyk2 locus. TYK2 was successfully removed by the use of ubiquitous and tissue-specific Cre-expressing mouse strains. Myeloid TYK2 was found to critically contribute to the defense against murine cytomegalovirus. Ubiquitous TYK2 ablation severely impaired tumor immunosurveillance, while deletion in myeloid, dendritic or T cells alone showed no effect. The conditional Tyk2 mouse strain will be instrumental to further dissect TYK2 functions in infection, inflammation and cancer.",

keywords = "Conditional knockout, JAK, Janus kinase, MC38, MCMV, Tumor transplant, Tyrosine kinase 2",

author = "Vielnascher, \{Raimund M.\} and Eva Hainzl and Leitner, \{Nicole R.\} and Michael Rammerstorfer and David Popp and Agnieszka Witalisz and Rita Rom and Marina Karaghiosoff and Thomas Kolbe and Simone M{\"u}ller and Thomas R{\"u}licke and Caroline Lassnig and Birgit Strobl and Mathias M{\"u}ller",

year = "2014",

month = jun,

doi = "10.1007/s11248-014-9795-y",

language = "English",

volume = "23",

pages = "519--529",

journal = "Transgenic Research",

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T1 - Conditional ablation of TYK2 in immunity to viral infection and tumor surveillance

AU - Vielnascher, Raimund M.

AU - Hainzl, Eva

AU - Leitner, Nicole R.

AU - Rammerstorfer, Michael

AU - Popp, David

AU - Witalisz, Agnieszka

AU - Rom, Rita

AU - Karaghiosoff, Marina

AU - Kolbe, Thomas

AU - Müller, Simone

AU - Rülicke, Thomas

AU - Lassnig, Caroline

AU - Strobl, Birgit

AU - Müller, Mathias

PY - 2014/6

Y1 - 2014/6

N2 - Tyrosine kinase 2 (TYK2) has a pivotal role in immunity to infection and tumor surveillance. It is associated with several cytokine receptor chains including type I interferon (IFN) receptor 1 (IFNAR1), interleukin- (IL-) 12 receptor beta 1 (IL-12Rb1) and IL-10R2. We have generated a mouse with a conditional Tyk2 null allele and proved integrity of the conditional Tyk2 locus. TYK2 was successfully removed by the use of ubiquitous and tissue-specific Cre-expressing mouse strains. Myeloid TYK2 was found to critically contribute to the defense against murine cytomegalovirus. Ubiquitous TYK2 ablation severely impaired tumor immunosurveillance, while deletion in myeloid, dendritic or T cells alone showed no effect. The conditional Tyk2 mouse strain will be instrumental to further dissect TYK2 functions in infection, inflammation and cancer.

AB - Tyrosine kinase 2 (TYK2) has a pivotal role in immunity to infection and tumor surveillance. It is associated with several cytokine receptor chains including type I interferon (IFN) receptor 1 (IFNAR1), interleukin- (IL-) 12 receptor beta 1 (IL-12Rb1) and IL-10R2. We have generated a mouse with a conditional Tyk2 null allele and proved integrity of the conditional Tyk2 locus. TYK2 was successfully removed by the use of ubiquitous and tissue-specific Cre-expressing mouse strains. Myeloid TYK2 was found to critically contribute to the defense against murine cytomegalovirus. Ubiquitous TYK2 ablation severely impaired tumor immunosurveillance, while deletion in myeloid, dendritic or T cells alone showed no effect. The conditional Tyk2 mouse strain will be instrumental to further dissect TYK2 functions in infection, inflammation and cancer.

KW - Conditional knockout

KW - JAK

KW - Janus kinase

KW - MC38

KW - MCMV

KW - Tumor transplant

KW - Tyrosine kinase 2

UR - https://www.scopus.com/pages/publications/84899974345

U2 - 10.1007/s11248-014-9795-y

DO - 10.1007/s11248-014-9795-y

M3 - Journal article

C2 - 24696087

AN - SCOPUS:84899974345

SN - 0962-8819

VL - 23

SP - 519

EP - 529

JO - Transgenic Research

JF - Transgenic Research

IS - 3

ER -

Conditional ablation of TYK2 in immunity to viral infection and tumor surveillance

Abstract

UN SDGs

Keywords

ASJC Scopus subject areas

Access to Document

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