Abstract
A broad understanding of the relationship between gene activation, pattern formation and morphogenesis will require adequate tools for three- dimensional and, perhaps four-dimensional, representation and analysis of molecular developmental processes. We present a novel, computer-based method for the 3D visualization of embryonic gene expression and morphological structures from serial sections. The information from these automatically aligned 3D reconstructions exceeds that from single-section and whole-mount visualizations of in situ hybridizations. In addition, these 3D models of gene-expression patterns can become a central component of a future developmental database designed for the collection and presentation of digitized, morphological and gene-expression data. This work is accompanied by a web site (http://www.univie.ac.at/GeneEMAC).
| Translated title of the contribution | Computergestützte dreidimensionale Visualisierung der Genexpression in der Entwicklung |
|---|---|
| Original language | English |
| Pages (from-to) | 147-152 |
| Number of pages | 6 |
| Journal | Nature Genetics |
| Volume | 25 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Jun 2000 |
| Externally published | Yes |
Keywords
- Anatomy, Cross-Sectional/methods
- Animals
- Automation
- Computer Simulation
- Databases, Factual
- Embryo, Mammalian/anatomy & histology
- Embryonic and Fetal Development
- Gene Expression Profiling/methods
- Gene Expression Regulation, Developmental
- Genetic Markers/genetics
- Image Processing, Computer-Assisted/methods
- In Situ Hybridization/methods
- Internet
- Mice
- Morphogenesis/genetics
- Organ Specificity
- Pattern Recognition, Automated
- Reproducibility of Results
- Sensitivity and Specificity
- Software
- Transcriptional Activation/genetics
ASJC Scopus subject areas
- Genetics