Basophil activation test shows high accuracy in the diagnosis of peanut and tree nut allergy: The Markers of Nut Allergy Study

Lucy Duan, Alper Celik, Jennifer A Hoang, Klara Schmidthaler, Delvin So, Xiaojun Yin, Christina M Ditlof, Marta Ponce, Julia E M Upton, Jean-Soo Lee, Lisa Hung, Heimo Breiteneder, Chiara Palladino, Adelle R Atkinson, Vy H D Kim, Alireza Berenjy, Maria Asper, David Hummel, Samantha Wong, Mara Alexanian-FarrAhuva Magder, Sharon R Chinthrajah, Kaori Mukai, Mindy Tsai, Kari Nadeau, Stephen J Galli, Arun K Ramani, Zsolt Szepfalusi, Thomas Eiwegger

Research output: Journal article (peer-reviewed)Journal article

33 Citations (Scopus)


BACKGROUND: Peanut and tree nut allergies are the most important causes of anaphylaxis. Co-reactivity to more than one nut is frequent, and co-sensitization in the absence of clinical data is often obtained. Confirmatory oral food challenges (OFCs) are inconsistently performed.

OBJECTIVE: To investigate the utility of the basophil activation test (BAT) in diagnosing peanut and tree nut allergies.

METHODS: The Markers Of Nut Allergy Study (MONAS) prospectively enrolled patients aged 0.5-17 years with confirmed peanut and/or tree nut (almond, cashew, hazelnut, pistachio, walnut) allergy or sensitization from Canadian (n = 150) and Austrian (n = 50) tertiary pediatric centers. BAT using %CD63+ basophils (SSClow/CCR3pos) as outcome was performed with whole blood samples stimulated with allergen extracts of each nut (0.001-1000 ng/mL protein). BAT results were assessed against confirmed allergic status in a blinded fashion to develop a generalizable statistical model for comparison to extract and marker allergen-specific IgE.

RESULTS: A mixed effect model integrating BAT results for 10 and 100 ng/mL of peanut and individual tree nut extracts was optimal. The area under the ROC curve (AUROC) was 0.98 for peanut, 0.97 for cashew, 0.92 for hazelnut, 0.95 for pistachio, and 0.97 for walnut. The BAT outperformed sIgE testing for peanut or hazelnut and was comparable for walnut (AUROC 0.95, 0.94, 0.92) in a sub-analysis in sensitized patients undergoing OFC.

CONCLUSIONS: Basophil activation test can predict allergic clinical status to peanut and tree nuts in multi-nut-sensitized children and may reduce the need for high-risk OFCs in patients.

Original languageEnglish
Pages (from-to)1800-1812
Number of pages13
JournalAllergy: European Journal of Allergy and Clinical Immunology
Issue number6
Publication statusPublished - Jun 2021
Externally publishedYes


  • Allergens
  • Arachis
  • Austria
  • Basophils
  • Canada
  • Child
  • Humans
  • Nut Hypersensitivity/diagnosis
  • Nuts
  • Peanut Hypersensitivity/diagnosis
  • Skin Tests


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