An infrared sensor system for the analysis and differentiation of living mammalian cells using D2O based microfluidics

R. Ebrahimifard, S. van den Driesche, H. Breiteneder, C. Hafner, M. J. Vellekoop

Research output: Journal article (peer-reviewed)Journal article

2 Citations (Scopus)

Abstract

In this study, we designed and realized a sensor platform using deuterium oxide (D2O) based microfluidics to allow in vitro infrared (IR) analysis of living cells. Using D2O instead of H2O based medium successfully resolved the high IR absorption issue of H2O at the 3.3–3.6 μm wavelength region. The spectra in this region contain the symmetric and antisymmetric CH2 and CH3 absorption, which can be used to monitor changes in the cells or the cell membranes. To demonstrate the performance and possible use of the system, IR analyses were conducted on three mammalian cell lines: Madin-Darby canine kidney (MDCK), rat basophilic cells (RS-ATL8), and aneuploid immortalized keratinocyte cells of adult human skin (HaCaT). The determination of the CH2 and CH3 absorption peaks allowed in vitro differentiation of the mammalian cell types. Successive viability tests showed normal cell proliferation in culture medium after incubation in D2O-PBS for 30 min. The IR spectra of fifteen separate measurements showed only small absorbance tolerances, which proved the high level of repeatability of the sensor system. This measurement system opens up the way to perform infrared cell analysis in liquid environments.

Original languageEnglish
Pages (from-to)981-991
Number of pages11
JournalSensors and Actuators, B: Chemical
Volume247
DOIs
Publication statusPublished - 2017

Keywords

  • Deuterium oxide
  • Infrared sensor
  • Mammalian cells analysis
  • Microfluidics

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Instrumentation
  • Condensed Matter Physics
  • Surfaces, Coatings and Films
  • Metals and Alloys
  • Electrical and Electronic Engineering
  • Materials Chemistry

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