TY - JOUR
T1 - Acid phosphatase test proves superior to standard phenotypic identification procedure for Clostridium perfringens strains isolated from water
AU - Ryzinska-Paier, G.
AU - Sommer, R.
AU - Haider, J. M.
AU - Knetsch, S.
AU - Frick, C.
AU - Kirschner, A. K.T.
AU - Farnleitner, A. H.
N1 - Funding Information:
This study was supported by the MA31 (project “Ground Water Resource Systems Vienna”) as well as by the FWF (project # P22309-B20 and # W1219-N22 “Vienna Doctoral Programme on Water Resource Systems” granted to A.H. Farnleitner). This is a joint publication of the InterUniversity Cooperation Centre Water and Health (ICC Water & Health).
PY - 2011/11
Y1 - 2011/11
N2 - Clostridium perfringens is used as an indicator for persistent faecal pollution as well as to monitor the efficacy of water treatment processes. For these purposes, differentiation between C. perfringens and other Clostridia is essential and is routinely carried out by phenotypic standard tests as proposed in the ISO/CD 6461-2:2002 (ISO_LGMN: lactose fermentation, gelatine liquidation, motility and nitrate reduction). Because the ISO_LGMN procedure is time consuming and labour intensive, the acid phosphatase test was investigated as a possible and much more rapid alternative method for confirmation. The aim of our study was to evaluate and compare confirmation results obtained by these two phenotypic methods using genotypically identified strains, what to our knowledge has not been accomplished before. For this purpose, a species specific PCR method was selected based on the results received for type strains and genotypically characterised environmental strains. For the comparative investigation type strains as well as presumptive C. perfringens isolates from water and faeces samples were used. The acid phosphatase test revealed higher percentage (92%) of correctly identified environmental strains (n = 127) than the ISO_LGMN procedure (83%) and proved to be a sensitive and reliable confirmation method.
AB - Clostridium perfringens is used as an indicator for persistent faecal pollution as well as to monitor the efficacy of water treatment processes. For these purposes, differentiation between C. perfringens and other Clostridia is essential and is routinely carried out by phenotypic standard tests as proposed in the ISO/CD 6461-2:2002 (ISO_LGMN: lactose fermentation, gelatine liquidation, motility and nitrate reduction). Because the ISO_LGMN procedure is time consuming and labour intensive, the acid phosphatase test was investigated as a possible and much more rapid alternative method for confirmation. The aim of our study was to evaluate and compare confirmation results obtained by these two phenotypic methods using genotypically identified strains, what to our knowledge has not been accomplished before. For this purpose, a species specific PCR method was selected based on the results received for type strains and genotypically characterised environmental strains. For the comparative investigation type strains as well as presumptive C. perfringens isolates from water and faeces samples were used. The acid phosphatase test revealed higher percentage (92%) of correctly identified environmental strains (n = 127) than the ISO_LGMN procedure (83%) and proved to be a sensitive and reliable confirmation method.
KW - Acid phosphatase test
KW - Clostridium perfringens
KW - Faecal pollution
KW - Genotypic confirmation
KW - Phenotypic confirmation
KW - Water
UR - http://www.scopus.com/inward/record.url?scp=80053570986&partnerID=8YFLogxK
U2 - 10.1016/j.mimet.2011.08.006
DO - 10.1016/j.mimet.2011.08.006
M3 - Journal article
C2 - 21872622
AN - SCOPUS:80053570986
SN - 0167-7012
VL - 87
SP - 189
EP - 194
JO - Journal of Microbiological Methods
JF - Journal of Microbiological Methods
IS - 2
ER -