A mouse model to identify cooperating signaling pathways in cancer

Monica Musteanu; Leander Blaas; Rainer Zenz; Jasmin Svinka; Thomas Hoffmann; Beatrice Grabner; Daniel Schramek; Hans-Peter Kantner; Mathias Müller; Thomas Kolbe; Thomas Rülicke; Richard Moriggl; Lukas Kenner; Dagmar Stoiber; Josef Martin Penninger; Helmut Popper; Emilio Casanova; Robert Eferl

doi:10.1038/nmeth.2130

A mouse model to identify cooperating signaling pathways in cancer

Monica Musteanu, Leander Blaas, Rainer Zenz, Jasmin Svinka, Thomas Hoffmann, Beatrice Grabner, Daniel Schramek, Hans-Peter Kantner, Mathias Müller, Thomas Kolbe, Thomas Rülicke, Richard Moriggl, Lukas Kenner, Dagmar Stoiber, Josef Martin Penninger, Helmut Popper, Emilio Casanova, Robert Eferl

Research output: Journal article (peer-reviewed) › Journal article

12 Citations (Scopus)

Abstract

We here establish a mouse cancer model called Multi-Hit that allows for the evaluation of oncogene cooperativities in tumor development. The model is based on the stochastic expression of oncogene combinations ('hits') that are mediated by Cre in a given tissue. Cells with cooperating hits are positively selected and give rise to tumors. We used this approach to evaluate the requirement of Ras downstream effector pathways in tumorigenesis.

Original language	English
Pages (from-to)	897-900
Number of pages	4
Journal	Nature Methods
Volume	9
Issue number	9
DOIs	https://doi.org/10.1038/nmeth.2130
Publication status	Published - Sept 2012
Externally published	Yes

Keywords

Animals
Disease Models, Animal
Female
Lung Neoplasms/genetics
Male
Mice
Mice, Transgenic
Neoplasms, Experimental/genetics
Oncogene Protein p21(ras)/genetics
Pancreatic Neoplasms/genetics
Signal Transduction/genetics

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10.1038/nmeth.2130

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Musteanu, M., Blaas, L., Zenz, R., Svinka, J., Hoffmann, T., Grabner, B., Schramek, D., Kantner, H.-P., Müller, M., Kolbe, T., Rülicke, T., Moriggl, R., Kenner, L., Stoiber, D., Penninger, J. M., Popper, H., Casanova, E., & Eferl, R. (2012). A mouse model to identify cooperating signaling pathways in cancer. Nature Methods, 9(9), 897-900. https://doi.org/10.1038/nmeth.2130

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author = "Monica Musteanu and Leander Blaas and Rainer Zenz and Jasmin Svinka and Thomas Hoffmann and Beatrice Grabner and Daniel Schramek and Hans-Peter Kantner and Mathias M{\"u}ller and Thomas Kolbe and Thomas R{\"u}licke and Richard Moriggl and Lukas Kenner and Dagmar Stoiber and Penninger, {Josef Martin} and Helmut Popper and Emilio Casanova and Robert Eferl",

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AU - Musteanu, Monica

AU - Blaas, Leander

AU - Zenz, Rainer

AU - Svinka, Jasmin

AU - Hoffmann, Thomas

AU - Grabner, Beatrice

AU - Schramek, Daniel

AU - Kantner, Hans-Peter

AU - Müller, Mathias

AU - Kolbe, Thomas

AU - Rülicke, Thomas

AU - Moriggl, Richard

AU - Kenner, Lukas

AU - Stoiber, Dagmar

AU - Penninger, Josef Martin

AU - Popper, Helmut

AU - Casanova, Emilio

AU - Eferl, Robert

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AB - We here establish a mouse cancer model called Multi-Hit that allows for the evaluation of oncogene cooperativities in tumor development. The model is based on the stochastic expression of oncogene combinations ('hits') that are mediated by Cre in a given tissue. Cells with cooperating hits are positively selected and give rise to tumors. We used this approach to evaluate the requirement of Ras downstream effector pathways in tumorigenesis.

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KW - Lung Neoplasms/genetics

KW - Male

KW - Mice

KW - Mice, Transgenic

KW - Neoplasms, Experimental/genetics

KW - Oncogene Protein p21(ras)/genetics

KW - Pancreatic Neoplasms/genetics

KW - Signal Transduction/genetics

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SP - 897

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JO - Nature Methods

JF - Nature Methods

IS - 9

ER -

A mouse model to identify cooperating signaling pathways in cancer

Abstract

Keywords

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