A Biomimetic, Silaffin R5-Based Antigen Delivery Platform

Daniela Reichinger; Manuel Reithofer; Mariam Hohagen; Mirjana Drinic; Joshua Tobias; Ursula Wiedermann; Freddy Kleitz; Beatrice Jahn-Schmid; Christian F.W. Becker

doi:10.3390/pharmaceutics15010121

A Biomimetic, Silaffin R5-Based Antigen Delivery Platform

Daniela Reichinger, Manuel Reithofer, Mariam Hohagen, Mirjana Drinic, Joshua Tobias, Ursula Wiedermann, Freddy Kleitz, Beatrice Jahn-Schmid, Christian F.W. Becker^*

^*Corresponding author for this work

Research output: Journal article (peer-reviewed) › Journal article

3 Citations (Scopus)

Abstract

Nature offers a wide range of evolutionary optimized materials that combine unique properties with intrinsic biocompatibility and that can be exploited as biomimetic materials. The R5 and RRIL peptides employed here are derived from silaffin proteins that play a crucial role in the biomineralization of marine diatom silica shells and are also able to form silica materials in vitro. Here, we demonstrate the application of biomimetic silica particles as a vaccine delivery and adjuvant platform by linking the precipitating peptides R5 and the RRIL motif to a variety of peptide antigens. The resulting antigen-loaded silica particles combine the advantages of biomaterial-based vaccines with the proven intracellular uptake of silica particles. These particles induce NETosis in human neutrophils as well as IL-6 and TNF-α secretion in murine bone marrow-derived dendritic cells.

Original language	English
Article number	121
Journal	Pharmaceutics
Volume	15
Issue number	1
DOIs	https://doi.org/10.3390/pharmaceutics15010121
Publication status	Published - 29 Dec 2022
Externally published	Yes

Keywords

adjuvant
biomaterial
biomineralization
silica particles

ASJC Scopus subject areas

Pharmaceutical Science

Access to Document

10.3390/pharmaceutics15010121

Cite this

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title = "A Biomimetic, Silaffin R5-Based Antigen Delivery Platform",

abstract = "Nature offers a wide range of evolutionary optimized materials that combine unique properties with intrinsic biocompatibility and that can be exploited as biomimetic materials. The R5 and RRIL peptides employed here are derived from silaffin proteins that play a crucial role in the biomineralization of marine diatom silica shells and are also able to form silica materials in vitro. Here, we demonstrate the application of biomimetic silica particles as a vaccine delivery and adjuvant platform by linking the precipitating peptides R5 and the RRIL motif to a variety of peptide antigens. The resulting antigen-loaded silica particles combine the advantages of biomaterial-based vaccines with the proven intracellular uptake of silica particles. These particles induce NETosis in human neutrophils as well as IL-6 and TNF-α secretion in murine bone marrow-derived dendritic cells.",

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author = "Daniela Reichinger and Manuel Reithofer and Mariam Hohagen and Mirjana Drinic and Joshua Tobias and Ursula Wiedermann and Freddy Kleitz and Beatrice Jahn-Schmid and Becker, {Christian F.W.}",

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doi = "10.3390/pharmaceutics15010121",

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volume = "15",

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AU - Reichinger, Daniela

AU - Reithofer, Manuel

AU - Hohagen, Mariam

AU - Drinic, Mirjana

AU - Tobias, Joshua

AU - Wiedermann, Ursula

AU - Kleitz, Freddy

AU - Jahn-Schmid, Beatrice

AU - Becker, Christian F.W.

PY - 2022/12/29

Y1 - 2022/12/29

N2 - Nature offers a wide range of evolutionary optimized materials that combine unique properties with intrinsic biocompatibility and that can be exploited as biomimetic materials. The R5 and RRIL peptides employed here are derived from silaffin proteins that play a crucial role in the biomineralization of marine diatom silica shells and are also able to form silica materials in vitro. Here, we demonstrate the application of biomimetic silica particles as a vaccine delivery and adjuvant platform by linking the precipitating peptides R5 and the RRIL motif to a variety of peptide antigens. The resulting antigen-loaded silica particles combine the advantages of biomaterial-based vaccines with the proven intracellular uptake of silica particles. These particles induce NETosis in human neutrophils as well as IL-6 and TNF-α secretion in murine bone marrow-derived dendritic cells.

AB - Nature offers a wide range of evolutionary optimized materials that combine unique properties with intrinsic biocompatibility and that can be exploited as biomimetic materials. The R5 and RRIL peptides employed here are derived from silaffin proteins that play a crucial role in the biomineralization of marine diatom silica shells and are also able to form silica materials in vitro. Here, we demonstrate the application of biomimetic silica particles as a vaccine delivery and adjuvant platform by linking the precipitating peptides R5 and the RRIL motif to a variety of peptide antigens. The resulting antigen-loaded silica particles combine the advantages of biomaterial-based vaccines with the proven intracellular uptake of silica particles. These particles induce NETosis in human neutrophils as well as IL-6 and TNF-α secretion in murine bone marrow-derived dendritic cells.

KW - adjuvant

KW - biomaterial

KW - biomineralization

KW - silica particles

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DO - 10.3390/pharmaceutics15010121

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ER -

A Biomimetic, Silaffin R5-Based Antigen Delivery Platform

Abstract

Keywords

ASJC Scopus subject areas

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