Tenascin-C promotes chronic pressure overload-induced cardiac dysfunction, hypertrophy and myocardial fibrosis

Bruno K Podesser, Maximilian Kreibich, Elda Dzilic, David Santer, Lorenz Förster, Sandra Trojanek, Dietmar Abraham, Martin Krššák, Klaus U Klein, Eva V Tretter, Christoph Kaun, Johann Wojta, Barbara Kapeller, Inês Fonseca Gonçalves, Karola Trescher, Attila Kiss

Publikation: Beitrag in Fachzeitschrift (peer-reviewed)Artikel in Fachzeitschrift

39 Zitate (Scopus)

Abstract

AIMS: Left ventricular (LV) hypertrophy is characterized by cardiomyocyte hypertrophy and interstitial fibrosis ultimately leading to increased myocardial stiffness and reduced contractility. There is substantial evidence that the altered expression of matrix metalloproteinases (MMP) and Tenascin-C (TN-C) are associated with the progression of adverse LV remodeling. However, the role of TN-C in the development of LV hypertrophy because of chronic pressure overload as well as the regulatory role of TN-C on MMPs remains unknown.

METHODS AND RESULTS: In a knockout mouse model of TN-C, we investigated the effect of 10 weeks of pressure overload using transverse aortic constriction (TAC). Cardiac function was determined by magnetic resonance imaging. The expression of MMP-2 and MMP-9, CD147 as well as myocardial fibrosis were assessed by immunohistochemistry. The expression of TN-C was assessed by RT-qPCR and ELISA. TN-C knockout mice showed marked reduction in fibrosis (P < 0.001) and individual cardiomyocytes size (P < 0.01), in expression of MMP-2 (P < 0.05) and MMP-9 (P < 0.001) as well as preserved cardiac function (P < 0.01) in comparison with wild-type mice after 10 weeks of TAC. In addition, CD147 expression was markedly increased under pressure overload (P < 0.01), irrespectively of genotype. TN-C significantly increased the expression of the markers of hypertrophy such as ANP and BNP as well as MMP-2 in H9c2 cells (P < 0.05, respectively).

CONCLUSION: Our results are pointed toward a novel signaling mechanism that contributes to LV remodeling via MMPs upregulation, cardiomyocyte hypertrophy as well as myocardial fibrosis by TN-C under chronic pressure overload.

OriginalspracheEnglisch
Seiten (von - bis)847-856
Seitenumfang10
FachzeitschriftJournal of Hypertension
Jahrgang36
Ausgabenummer4
DOIs
PublikationsstatusVeröffentlicht - Apr. 2018

ASJC Scopus Sachgebiete

  • Kardiologie und kardiovaskuläre Medizin
  • Physiologie
  • Innere Medizin

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