Abstract
A quantitative TaqMan minor-groove binder real-time PCR assay was developed for the sensitive detection of a ruminant-specific genetic marker in fecal members of the phylum Bacteroidetes. The qualitative and quantitative detection limits determined were 6 and 20 marker copies per PCR, respectively. Tested ruminant feces contained an average of 4.1 × 109 marker equivalents per g, allowing the detection of 1.7 ng of feces per filter in fecal suspensions. The marker was detected in water samples from a karstic catchment area at levels matching a gradient from negligible to considerable ruminant fecal influence (from not detectable to 105 marker equivalents per liter).
Originalsprache | Englisch |
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Seiten (von - bis) | 5610-5614 |
Seitenumfang | 5 |
Fachzeitschrift | Applied and Environmental Microbiology |
Jahrgang | 72 |
Ausgabenummer | 8 |
DOIs | |
Publikationsstatus | Veröffentlicht - Aug. 2006 |
Extern publiziert | Ja |
ASJC Scopus Sachgebiete
- Biotechnologie
- Lebensmittelwissenschaften
- Angewandte Mikrobiologie und Biotechnologie
- Ökologie