Quantitative PCR method for sensitive detection of ruminant fecal pollution in freshwater and evaluation of this method in alpine karstic regions

Georg H. Reischer, David C. Kasper, Ralf Steinborn, Robert L. Mach, Andreas H. Farnleitner*

*Korrespondierende:r Autor:in für diese Arbeit

Publikation: Beitrag in Fachzeitschrift (peer-reviewed)Artikel in Fachzeitschrift

159 Zitate (Scopus)

Abstract

A quantitative TaqMan minor-groove binder real-time PCR assay was developed for the sensitive detection of a ruminant-specific genetic marker in fecal members of the phylum Bacteroidetes. The qualitative and quantitative detection limits determined were 6 and 20 marker copies per PCR, respectively. Tested ruminant feces contained an average of 4.1 × 109 marker equivalents per g, allowing the detection of 1.7 ng of feces per filter in fecal suspensions. The marker was detected in water samples from a karstic catchment area at levels matching a gradient from negligible to considerable ruminant fecal influence (from not detectable to 105 marker equivalents per liter).

OriginalspracheEnglisch
Seiten (von - bis)5610-5614
Seitenumfang5
FachzeitschriftApplied and Environmental Microbiology
Jahrgang72
Ausgabenummer8
DOIs
PublikationsstatusVeröffentlicht - Aug. 2006
Extern publiziertJa

ASJC Scopus Sachgebiete

  • Biotechnologie
  • Lebensmittelwissenschaften
  • Angewandte Mikrobiologie und Biotechnologie
  • Ökologie

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