TY - JOUR
T1 - Impact of maternally derived meiotic aneuploidies on early embryonic development in vitro
AU - Tschare, Lena
AU - Ennemoser, A
AU - Carli, L
AU - Vaccari, E
AU - Feichtinger, M
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023/11
Y1 - 2023/11
N2 - Purpose: To assess early embryonic developmental potential of embryos affected by maternally inherited meiotic aneuploidies. Methods: This observational, descriptive study includes 930 oocytes from 151 patients which were retrospectively analyzed by combining the morphological assessment with the genetic results from polar body diagnosis. Results: Of 930 oocytes examined, 566 (60.9%) were tested aneuploid. Developmental potential until cleavage stage was not affected by trisomies or monosomies (69.6% vs. 77.1%, p = 0.75). However, trisomies significantly more often resulted in top quality cleavage stage embryos compared to monosomies (20% vs. 17.6%, p = < 0.01). Top quality blastocysts were more likely to be euploid than aneuploid (52.4% vs. 47.6%, p = 0.032). Additionally, significantly more aneuploid embryos resulted in developmental arrest compared to euploid embryos (15.3% vs. 6.7%, p = 0.003). Overall, there was no significant difference in the frequency of trisomies and monosomies in blastocyst stage embryos. (28.3% vs. 28.2%; p = 0.81). In contrast to earlier developmental stages, distribution of trisomies and monosomies did not differ in top quality blastocysts (8.3% vs. 5.3%, p = 0.32). However, certain chromosomal abnormalities showed a higher potential to develop into a top-rated blastocyst. These included monosomies 2, 5, 8, 10, 16, 17, 20, 21, and 22 and trisomies 2, 4, 5, 8, 9, 10, 11, 12, 13, 16, 17, 18 and 20. Conclusion: Meiotically induced maternal aneuploidies have different effects on early embryonic development. While no difference in developmental potential between monosomies and trisomies could be observed in blastocysts, cleavage stage quality was significantly affected by chromosomal aneuploidies.
AB - Purpose: To assess early embryonic developmental potential of embryos affected by maternally inherited meiotic aneuploidies. Methods: This observational, descriptive study includes 930 oocytes from 151 patients which were retrospectively analyzed by combining the morphological assessment with the genetic results from polar body diagnosis. Results: Of 930 oocytes examined, 566 (60.9%) were tested aneuploid. Developmental potential until cleavage stage was not affected by trisomies or monosomies (69.6% vs. 77.1%, p = 0.75). However, trisomies significantly more often resulted in top quality cleavage stage embryos compared to monosomies (20% vs. 17.6%, p = < 0.01). Top quality blastocysts were more likely to be euploid than aneuploid (52.4% vs. 47.6%, p = 0.032). Additionally, significantly more aneuploid embryos resulted in developmental arrest compared to euploid embryos (15.3% vs. 6.7%, p = 0.003). Overall, there was no significant difference in the frequency of trisomies and monosomies in blastocyst stage embryos. (28.3% vs. 28.2%; p = 0.81). In contrast to earlier developmental stages, distribution of trisomies and monosomies did not differ in top quality blastocysts (8.3% vs. 5.3%, p = 0.32). However, certain chromosomal abnormalities showed a higher potential to develop into a top-rated blastocyst. These included monosomies 2, 5, 8, 10, 16, 17, 20, 21, and 22 and trisomies 2, 4, 5, 8, 9, 10, 11, 12, 13, 16, 17, 18 and 20. Conclusion: Meiotically induced maternal aneuploidies have different effects on early embryonic development. While no difference in developmental potential between monosomies and trisomies could be observed in blastocysts, cleavage stage quality was significantly affected by chromosomal aneuploidies.
KW - Aneuploidies
KW - IVF
KW - Polar bodies
KW - PGT-A
KW - Embryo quality
KW - Trisomy
KW - Humans
KW - Blastocyst
KW - Aneuploidy
KW - Pregnancy
KW - Embryonic Development/genetics
KW - Preimplantation Diagnosis/methods
KW - Female
KW - Retrospective Studies
KW - Monosomy
UR - http://www.scopus.com/inward/record.url?scp=85169087724&partnerID=8YFLogxK
U2 - 10.1007/s10815-023-02922-9
DO - 10.1007/s10815-023-02922-9
M3 - Journal article
C2 - 37632639
SN - 1058-0468
VL - 40
SP - 2715
EP - 2723
JO - Journal of Assisted Reproduction and Genetics
JF - Journal of Assisted Reproduction and Genetics
IS - 11
ER -