Computer-based three-dimensional visualization of developmental gene expression

J Streicher; M A Donat; B Strauss; R Spörle; K Schughart; G B Müller

doi:10.1038/75989

Computer-based three-dimensional visualization of developmental gene expression

Titel in Übersetzung: Computergestützte dreidimensionale Visualisierung der Genexpression in der Entwicklung

J Streicher
, M A Donat
, B Strauss
, R Spörle
, K Schughart
, G B Müller

Publikation: Beitrag in Fachzeitschrift (peer-reviewed) › Artikel in Fachzeitschrift

76 Zitate (Scopus)

Abstract

Ein umfassendes Verständnis der Beziehung zwischen Genaktivierung, Musterbildung und Morphogenese erfordert geeignete Werkzeuge für die dreidimensionale und vielleicht vierdimensionale Darstellung und Analyse molekularer Entwicklungsprozesse. Wir stellen eine neuartige, computergestützte Methode zur 3D-Visualisierung der embryonalen Genexpression und der morphologischen Strukturen von Serienschnitten vor. Die Informationen aus diesen automatisch ausgerichteten 3D-Rekonstruktionen übertreffen die Informationen aus Einzelschnitten und Ganzkörper-Visualisierungen von In-situ-Hybridisierungen.

Titel in Übersetzung	Computergestützte dreidimensionale Visualisierung der Genexpression in der Entwicklung
Originalsprache	Englisch
Seiten (von - bis)	147-152
Seitenumfang	6
Fachzeitschrift	Nature Genetics
Jahrgang	25
Ausgabenummer	2
DOIs	https://doi.org/10.1038/75989
Publikationsstatus	Veröffentlicht - Juni 2000
Extern publiziert	Ja

ASJC Scopus Sachgebiete

Genetik

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10.1038/75989

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title = "Computer-based three-dimensional visualization of developmental gene expression",

abstract = "A broad understanding of the relationship between gene activation, pattern formation and morphogenesis will require adequate tools for three- dimensional and, perhaps four-dimensional, representation and analysis of molecular developmental processes. We present a novel, computer-based method for the 3D visualization of embryonic gene expression and morphological structures from serial sections. The information from these automatically aligned 3D reconstructions exceeds that from single-section and whole-mount visualizations of in situ hybridizations. In addition, these 3D models of gene-expression patterns can become a central component of a future developmental database designed for the collection and presentation of digitized, morphological and gene-expression data. This work is accompanied by a web site (http://www.univie.ac.at/GeneEMAC).",

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year = "2000",

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AU - Streicher, J

AU - Donat, M A

AU - Strauss, B

AU - Spörle, R

AU - Schughart, K

AU - Müller, G B

PY - 2000/6

Y1 - 2000/6

N2 - A broad understanding of the relationship between gene activation, pattern formation and morphogenesis will require adequate tools for three- dimensional and, perhaps four-dimensional, representation and analysis of molecular developmental processes. We present a novel, computer-based method for the 3D visualization of embryonic gene expression and morphological structures from serial sections. The information from these automatically aligned 3D reconstructions exceeds that from single-section and whole-mount visualizations of in situ hybridizations. In addition, these 3D models of gene-expression patterns can become a central component of a future developmental database designed for the collection and presentation of digitized, morphological and gene-expression data. This work is accompanied by a web site (http://www.univie.ac.at/GeneEMAC).

AB - A broad understanding of the relationship between gene activation, pattern formation and morphogenesis will require adequate tools for three- dimensional and, perhaps four-dimensional, representation and analysis of molecular developmental processes. We present a novel, computer-based method for the 3D visualization of embryonic gene expression and morphological structures from serial sections. The information from these automatically aligned 3D reconstructions exceeds that from single-section and whole-mount visualizations of in situ hybridizations. In addition, these 3D models of gene-expression patterns can become a central component of a future developmental database designed for the collection and presentation of digitized, morphological and gene-expression data. This work is accompanied by a web site (http://www.univie.ac.at/GeneEMAC).

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KW - Animals

KW - Automation

KW - Computer Simulation

KW - Databases, Factual

KW - Embryo, Mammalian/anatomy & histology

KW - Embryonic and Fetal Development

KW - Gene Expression Profiling/methods

KW - Gene Expression Regulation, Developmental

KW - Genetic Markers/genetics

KW - Image Processing, Computer-Assisted/methods

KW - In Situ Hybridization/methods

KW - Internet

KW - Mice

KW - Morphogenesis/genetics

KW - Organ Specificity

KW - Pattern Recognition, Automated

KW - Reproducibility of Results

KW - Sensitivity and Specificity

KW - Software

KW - Transcriptional Activation/genetics

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EP - 152

JO - Nature Genetics

JF - Nature Genetics

IS - 2

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Computer-based three-dimensional visualization of developmental gene expression

Abstract

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