Automatic segmentation of skin cells in multiphoton data using multi-stage merging

Philipp Prinke, Jens Haueisen, Sascha Klee, Muhammad Qurhanul Rizqie, Eko Supriyanto, Karsten König, Hans Georg Breunig, Łukasz Piątek

Publikation: Beitrag in Fachzeitschrift (peer-reviewed)Artikel in Fachzeitschrift

5 Zitate (Scopus)

Abstract

We propose a novel automatic segmentation algorithm that separates the components of human skin cells from the rest of the tissue in fluorescence data of three-dimensional scans using non-invasive multiphoton tomography. The algorithm encompasses a multi-stage merging on preprocessed superpixel images to ensure independence from a single empirical global threshold. This leads to a high robustness of the segmentation considering the depth-dependent data characteristics, which include variable contrasts and cell sizes. The subsequent classification of cell cytoplasm and nuclei are based on a cell model described by a set of four features. Two novel features, a relationship between outer cell and inner nucleus (OCIN) and a stability index, were derived. The OCIN feature describes the topology of the model, while the stability index indicates segment quality in the multi-stage merging process. These two new features, combined with the local gradient magnitude and compactness, are used for the model-based fuzzy evaluation of the cell segments. We exemplify our approach on an image stack with 200 × 200 × 100 μm3, including the skin layers of the stratum spinosum and the stratum basale of a healthy volunteer. Our image processing pipeline contributes to the fully automated classification of human skin cells in multiphoton data and provides a basis for the detection of skin cancer using non-invasive optical biopsy.

OriginalspracheEnglisch
Aufsatznummer14534
Seiten (von - bis)14534
FachzeitschriftScientific Reports
Jahrgang11
Ausgabenummer1
DOIs
PublikationsstatusVeröffentlicht - Dez. 2021

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